对蜈蚣藻中萜类化合物进行提取、分离纯化和GC-MS分析。采用正交试验研究超临界C02流体萃取蜈蚣藻中萜类化合物的条件,并用气相色谱-质谱(GC-MS)联用技术分析蜈蚣藻中萜类化合物的主要化学成分。正交试验结果显示:以得油量为指标,各萃取条件按对结果影响的顺序列为萃取压力〉萃取时间〉萃取温度=CO2流速;最佳萃取条件为萃取压力30MPa、萃取时间2h、萃取温度40℃、CO2流速10L/h。各浓度洗脱液经GC-MS分析后,通过检索NIST谱图库,并结合有关文献,从中初步确认出11种萜类化合物。
The extraction, purification and GC-MS analysis of terpenoid compounds from Grateloupiafilicina C.Ag. were investigated in the present study. The supercritical fluid extraction conditions of terpenoid compounds from Grateloupiafilicina C.Ag. were optimized by orthogonal test, and the main components in the terpenoid compounds were analyzed by GC-MS. The results of orthogonal test showed that the extraction pressure presented the most significant effect on yield of terpenoid compounds, followed by extraction duration, temperature and flow rate in turn, and the optimum extraction conditions were as follows: with flow rate of 10 L/h, extraction at 30 MPa and 40 ℃ for 2 h. By searching in NIST98 spectrum library and refering to related literature, finally 11 kinds of terpenoid compounds were confirmed from the extract of Grateloupiafilicina C.Ag.