中文摘要：

结合表达谱数据分析,参考公共数据库中的序列信息设计引物,利用RT-PCR技术,从东乡野生稻中获得了1个在低温诱导条件下高表达的螺旋—环—螺旋结构域蛋白基因BGIOSGA013293-DX的全长c DNA,并构建了其过表达载体。序列比对分析表明,该基因位点在93-11中编码308个氨基酸,而在东乡野生稻中编码335个氨基酸,比93-11多27个氨基酸。且这27个氨基酸连续分布在一起,另外还有1个氨基酸的差异。利用农杆菌介导法将BGIOSGA013293-DX转入水稻受体品种93-11,获得了9株过表达转基因水稻植株,经潮霉素抗性标记基因PCR阳性检测和GUS检测,获得的转基因植株为携带BGIOSGA013293-DX的阳性植株。

英文摘要：

Based on the analysis of expression profile data, the full length cDNA of BGIOSGAO13293 - DX gene en- coding helix-loop-helix protein with a high expression after cold treatment was cloned from the cold-tolerant Dongx- iang wild rice by reverse transcription polymerase chain reaction （RT -PCR） with the primers designed on the ba- sis of sequence information in the public database, and its over-expression vector was then constructed. The se- quence alignment analysis showed that there were 335 amino acids encoded by the gene in Dongxiang wild rice while there were only 308 amino acids encoded by the gene in 93-11. The extra 27 amino acids in Dongxiang wild rice were linked together with their serial No. from 187 to 213. The other 308 amino acids were the same except for No. 81 amino acid between 93-11 and Dongxiang wild rice. By the agrobacterium-mediated method, the gene BGIOSGA013293 -DX was transformed into the receptor rice variety 93-11. Nine over-expression transgenic rice plants were obtained. After the PCR detection of hygromycin resistance gene and GUS test, they were found to be positive transgenic plants carrying BGIOSGA013293 -DX.