中文摘要：

目的通过建立炎症相关性结直肠癌小鼠模型,探讨吴茱萸碱在炎症相关性结直肠癌中的预防作用。方法用AOM/DSS建立炎症相关性结直肠癌模型,将小鼠分为空白组、模型组、吴茱萸碱低剂量组（10mg/kg）和吴茱萸碱高剂量组（20mg/kg）,给药过程中记录各组小鼠体质量、死亡情况,比较各组小鼠结直肠的长度、肿瘤数目及大小,通过HE染色观察各组小鼠的结直肠组织形态,通过免疫组化法检测COX-2和PCNA的表达。结果 AOM/DSS可造成小鼠的体质量下降和死亡率增多,而吴茱萸碱可改善这一现象,并且减少结直肠肿瘤的发生,降低组织炎症水平和细胞的异常增殖。结论吴茱萸碱对炎症相关性结直肠癌具有良好的预防作用,其作用可能与降低组织炎症水平和抑制细胞异常增殖有关。

英文摘要：

ABSTRACT：OBJECTIVE To explore the preventive effect of evodiamine on inflammation associated colorectal carcinoma through the establishment of model in mice.METHODS The mice were administrated with AOM/DSS and divided into con-trol group,model group,low dose of evodiamine group（10EV）and high dose of evodiamine group（20EV）.Body weights and death rates of mice were recorded once a week.The length of colorectum,tumor number and sizes were compared among all groups.Morphology of colorectal tissues in each group were stained by HE.Immunohistochemical analysis was used to detect the expression of COX-2 and PCNA.RESULTS AOM/DSS caused the loss of body weight and increased mortality of mice, while evodiamine improved the condition,and also reduced the development of colorectal cancer,alleviated the inflammatory response and inhibited abnormal proliferation of cancer cells.CONCLUSION Evodiamine can display promising preventive effects on inflammation-associated colorectal cancer,which is relative to the reduction of inflammation and abnormal cell prolif-eration.