中文摘要：

目的 鉴定局灶性脑缺血相关蛋白并筛选早期神经保护蛋白.方法 应用先进的差异蛋白质组学荧光差异双向凝胶电泳（2D DIGE）技术,比较大鼠大脑中动脉闭塞（MCAO）6 h病灶侧大脑皮层和正常大鼠相应部位蛋白质变化;采用DeCyder-DIA软件、单因素方差分析ANOVA,选择两组间蛋白表达量差异具有统计学意义（P＜0.05）且AR＞1.4的蛋白点;基质辅助激光解析/电离-飞行时间（MALDI-TOF）质谱鉴定差异蛋白.结果 脑缺血6 h组与正常对照组比较13个蛋白点符合统计学要求;经质谱分析仅鉴定出一个缺血组明显减少的蛋白点为α-微管蛋白.结论 作为结构蛋白之一的α-微管蛋白在脑缺血早期即发生明显变化,是缺血性脑血管病早期相关蛋白.

英文摘要：

Objective To identify cerebral ischemia-relevant proteins and to screen early neuron-protection protein. Methods A comparative proteomic approach, fluorescence two-dimensional differential in-gel electrophoresis （2D DIGE） was used to compare the changes in protein between the left hemisphere cerebral cortex tissues following 6 h middle cerebral artery occlusion （MCAO） and the corresponding sites of healthy control rats. Protein spots expression levels showed statistically significant （ P〈0.05 ） and AR 〉1.4 on every gel were selected by DeCyder differential in-gel analysis （DIA） software and one-way analysis of variance （ANOVA）. Peptide mass fingerprinting was used by matrix-assisted laser desorption/ionization-time of flight （MALDI-TOF） mass spectrometry to identify all difference protein spots. Results There were 13 protein spots consistent with statistical requests in Cy3 and Cy5 labeled samples between ischemia 6 h group and control group. Only one protein was identified as α-tubulin significantly decreased in the ischemia rats. Conclusions α-tubulin as one of the cytoskeletal proteins whose expression levels are decreased in the early stage after MCAO focal ischemia, which is related proteins of cerebral ischemia at early stage.