中文摘要：

通过分析改良的Hoagland营养液中添加50-400μmol·L^-1Al Cl3处理铝耐受型黑大豆RB和铝敏感型黑大豆SB对NO-3吸收量与根中H2O2含量、质膜H＋-ATP酶磷酸化水平及其与14-3-3蛋白的互作、质膜H＋-ATP酶活性及H＋泵活性的相关性来阐明铝胁迫下黑大豆根吸收NO-3的机理。结果表明：铝胁迫显著降低RB和SB对硝态氮吸收量,在无铝胁迫和铝胁迫第一天,SB的硝态氮吸收量约为RB的4倍,但随着铝胁迫浓度的增加和铝胁迫时间的延长,在每个处理中RB的硝态氮吸收量反超SB一倍以上。在正常情况下,RB根中H2O2含量比SB高,100μmol·L^-1铝胁迫使SB根中H2O2含量比RB高约4倍,使RB根中的质膜H＋-ATPase活性和H＋泵活性分别比SB高约7倍和10倍。Co-IP分析证明100μmol·L-1铝胁迫诱导RB根中质膜H＋-ATPase磷酸化水平及其与14-3-3蛋白相互作用,但在SB根中起抑制作用。铝胁迫可能通过改变根中积累的H2O2来影响RB和SB的质膜H＋-ATPase的磷酸化水平,从而改变磷酸化质膜H＋-ATP酶与14-3-3蛋白的相互作用,进而影响RB和SB根的质膜H＋-ATP酶活性和H＋泵活性,最终表现为RB和SB硝态氮吸收量的不同。

英文摘要：

The mechanism of Al stress on NO3^- uptake in the two types of black soybean would be clarified by studying the correlation among the NO-3uptake,H2O2 content,and phosphorylation level of PM H^＋-ATPase as well as its interaction with14-3-3 proteins and the PM H^＋-ATPase activity and H＋pump activity in the root tips of RB and SB in improved Hoagland solution containing 50,100,200,400 μmol·L^- 1Al. The results indicated that Al stress significantly reduced the amount of NO-3uptake by RB and SB. On the first day of non-and Al-stress conditions,the NO-3uptake by SB was approximately four times than that of RB. However,with the increase of stress concentration and time,the amount of NO-3uptake by RB in each treatment was almost 2-fold greater than that by SB. Under normal conditions,the H2O2 content in the RB root tips was greater than SB. Under 100 μmol·L^- 1Al stress,the H2O2 content in the SB root tips was approximately four times greater than RB root tips. The PM H^＋-ATPase and H＋pump activities in the RB root tips were approximately 7-fold and 10-fold greater than those in SB root tips,respectively. Co-IP analysis confirmed that 100 μmol·L^- 1Al stress induced the phosphorylation of PM H^＋-ATPase and the interaction between PM H＋-ATPase and 14-3-3 proteins in RB root tips,but the opposite results appeared in the SB root tips. In conclusion,Al stress might affect the phosphorylation level of PM H^＋-ATPase by altering the H2O2 accumulation in the RB and SB root tips,further affect the interaction between PM H^＋-ATPase phosphorylation and 14-3-3 proteins,and change the PM H^＋-ATP and H＋pump activities. Consequently,the NO3^-uptake by RB and SB become different.