中文摘要：

目的：明确小鼠透明带ZP2蛋白的细胞来源。方法：采用组织原位杂交技术检测小鼠卵泡中的ZP2mRNA的表达,通过灰度测量比较小鼠ZP2mRNA在小鼠不同阶段卵泡颗粒层细胞的表达。结果：小鼠ZP2RNA探针与小鼠各级卵泡都有杂交信号,而在卵泡的颗粒细胞也有明显信号,颗粒细胞层的信号强度随卵泡发育而不同,初级卵泡的颗粒细胞层信号最弱,腔囊卵泡的颗粒细胞层信号最强。结论：小鼠ZP2mRNA在小鼠卵泡颗粒细胞层也表达,而非卵母细胞特异产生。

英文摘要：

Aim：This work was aimed at illuminating the cellular localization of biosynthesis of mZP2. Methods： In situ hybridization was carried out to detect the mRNA of ZP2 in mouse follicles,especially in granulose cells.Grey levels were measured to quantitate the ZP2 mRNA in the granulose cells among follicles at different stages.Results： Results showed that hybridization of ZP2 mRNA was detected in the mouse follicles at different stages,including the granulose cells.The signals of hybridization in the granulose cells varied as the follicle developed,with the lowest in the primary follicle and the highest in the antral follicle.Conclusion：These data indicate that the mRNA of mouse ZP2 is expressed not only in oocyte exclusively,but also in granlose cells.