目的探讨海河流域诺如病毒与新型指示微生物——通用拟杆菌的相关性。方法于2013年12月—2014年11月对天津海河流域水中的粪大肠菌群、F(+)噬菌体、通用拟杆菌3项指示微生物和沙门菌、诺如病毒2项致病微生物进行监测。结果粪大肠菌群浓度为0~6.85×10^4 CFU/100 ml,F(+)噬菌体浓度为0~7.70×10^3 PFU/L,通用拟杆菌浓度为0~6.07×10^6copies/ml,沙门氏菌检出率为30.95%,诺如病毒检出率为27.38%。统计学分析显示,粪大肠菌群与沙门菌具有相关性(r=0.243,P〈0.05),与诺如病毒没有相关性;通用拟杆菌与诺如病毒具有相关性(r=0.289,P〈0.01),与沙门菌没有相关性;F(+)噬菌体与沙门菌和诺如病毒均没有相关性。结论海河流域中诺如病毒与通用拟杆菌具有相关性,而与粪大肠菌群无相关性。
Objective To investigate the correlation between norovirus and novel indicator microorganism in Haihe Rive.Methods In this one-year(Dec. 2013-Nov. 2014) field investigation, water samples were collected from Haihe River Basin in Tianjin. Three indicator microorganisms, fecal coliform, universal bacteroides, F(+) bacteriophage, and two pathogenic microbes,norovirus, Salmonella, were detected in the water samples. Results The concentration of fecal coliform ranged from 0 to 6.85×10^4CFU/100 ml, the concentration of F( +) bacteriophage ranged from 0 to 7. 70 × 10^3PFU/L and universal bacteroides concentration ranged from 0 to 6.07 ×10^6copies/ml. In addition, the detection rates of Salmonella and norovirus in the water samples were 30.95% and 27.38%, respectively. According to the statistical analysis, the fecal coliform was associated with the presence of Salmonella(r =0.243, P〈0.05), but it had no correlation with the occurrence of norovirus. On the contrary, the occurrence of universal bacteroides was correlated with norovirus(r =0.289, P〈0.01), but it was not associated with the Salmonella. The presence of F( +) bacteriophage and Salmonella had no correlation with the occurrence of the norovirus.Conclusion Norovirus were correlated with universal bacteroides in Haihe River, and no correlation with fecal coliform.