中文摘要：

Nanozymes 由于容易的准备和低费用的优点收到了大注意。不同于乐意地适应生理的环境的自然的酶，人工的 nanozymes 是容易的在复杂临床的样品使钝化(例如，浆液) ，它可以损坏催化能力并且因而在生物医学的分析限制申请。为了征服这个问题，在这研究，我们由把 nanozymes 合并到纯 DNA hydrogel 制作了新奇 nanozyme@DNA hydrogel 建筑学。金 nanoparticles (AuNPs ) 作为模型 nanozyme 被采用。当他们被 hydrogel 保护，结果显示在 DNA hydrogel 合并的 AuNPs 在浆液保留他们的催化能力，而 AuNPs 完全独自在浆液失去催化能力。在浆液的氢过氧化物和葡萄糖的察觉基于 AuNPs@DNA hydrogel 的催化作用被完成。分别地，各个的察觉限制到达 1.7 和 38 M 它等于用自然的酶获得的价值。除机制以外，一些另外的优点例如再循环能力和可获得性，也被探索了。这 nanozyme@DNA hydrogel 体系结构可以在复杂生理的样品为生物医学的分析象 nanozymes 的申请一样为 nanozymes 的利用有一个大潜力。

英文摘要：

Nanozymes have received great attention owing to the advantages of easy preparation and low cost. Unlike natural enzymes that readily adapt to physiological environments, artificial nanozymes are apt to passivate in complex clinical samples （e.g., serum）, which may damage the catalytic capability and consequently limit the application in biomedical analysis. To conquer this problem, in this study, we fabricated novel nanozyme@DNA hydrogel architecture by incorporat~ng nanozymes into a pure DNA hydrogel. Gold nanoparticles （AuNPs） were adopted as a model nanozyme. Results indicate that AuNPs incorporated in the DNA hydrogel retain their catalytic capability in serum as they are protected by the hydrogel, whereas AuNPs alone totally lose the catalytic capability in serum. The detection of hydrogen peroxide and glucose in serum based on the catalysis of the AuNPs@DNA hydrogel was achieved. The detection limit of each reaches 1.7 and 38 ~M, respectively, which is equal to the value obtained using natural enzymes. Besides the mechanisms, some other advantages, such as recyclability and availability, have also been explored. This nanozyme@DNA hydrogel architecture may have a great potential for the utilization of nanozymes as well as the application of nanozymes for biomedical analysis in complex physiological samples.