中文摘要：

脂肪酸合酶（FAS）是治疗肥胖症的潜在靶点.实验测定表明,草莓果肉渣及草莓叶的乙醇提取物再经乙酸乙酯萃取的有效组分对FAS具有强抑制作用,且分别对FAS的底物丙二酸单酰辅酶A、乙酰辅酶A呈非竞争性和竞争性抑制.经HPLC-MS分析,槲皮素和鞣花酸可能是草莓有效组分中起主要作用的物质.草莓叶及果肉渣乙酸乙酯提取组分均可有效抑制3T3-L1前脂肪细胞中脂滴积累,且呈剂量依赖性.实验结果对于研究草莓在防治肥胖症的应用上可能具有重要价值.

英文摘要：

Fatty acid synthase （FAS） has been considered as a potential new therapeutic target for obesity.In the present study we show that the components of both strawberry （Fragaria ananassa Duch） fruit dreg and strawberry leaves that extracted with 50% ethanol and partitioned by ethyl acetate are potent FAS inhibitors.The ethyl acetate fractions of strawberry fruit dregs （SF-AE） show noncompetitive inhibition with the substrate MalCoA,while the ethyl acetate fractions of strawberry leaves （SL-AE） show competitive inhibition with Ac-CoA.Based on the HPLC-MS analysis,we conclude that ellagic acid and quercetin are the efficient components.SFAE and SL-AE could reduce lipids accumulation in 3T3-L1 preadipocyte with dose-dependence.These results may be of great value for application of strawberry extracts to obesity prevention and cure.