中文摘要：

目的：研究针刺治疗骨骼肌过度使用损伤的疗效。方法：18只雄性SD大鼠随机分成安静对照组（C）、1周自然恢复组（1RG）和1周针刺恢复组（1RAG）3组。通过4周低强度离心运动方式建立过度使用损伤动物模型（动物跑台、一160、60．90分钟／天，5天／周）。恢复1周后，在第5周取股中肌。采用透射电子显微镜观察骨骼肌细胞超微结构损伤累积；采用扫描电子显微镜观察骨骼肌神经、血管结构损伤和胶原纤维结构变化；采用免疫组化方法量化沉积的胶原纤维中collagenI纤维蛋白浓度。结果：l周自然恢复组呈现过度使用损伤，包括Z线水波纹样变化，肌原纤维间隙变宽，肌原纤维降解．肌膜下线粒体聚积，肌纤维内线粒体降解物聚积，肌肉内神经和血管纤维化，以及肌内膜尤其是肌束膜厚度增加、collagenI纤维蛋白浓度增加。针刺显著减轻了骨骼肌过度使用损伤（P〈0．01）。包括肌原纤维降解减少，骨骼肌超微结构损伤修复，线粒体数量增加，骨骼肌神经和血管结构损伤减弱．以及肌内膜尤其是肌束膜的厚度减少、collagenI纤维蛋白浓度下调。结论：针刺能治疗离心运动引起的骨骼肌过度使用损伤，其效应-9线粒体数量增加有关。

英文摘要：

Objective The aim of this study was to investigate the effects of acupuncture therapy on the eccentric exercise-induced skeletal muscle overuse injuries. Methods Eighteen male Sprague- Dawley rats were assigned randomly to three groups ：the control group （C）, 1 week recovery group （1 RG）and 1 week received intramuscular acupuncture recovery group （1 RAG）. Animal model of overuse injury was experimentally induced through 4-week low-intensity eccentric exercise （treadmill running, -16~ ,60 ~90 min/day,5 days/week）. After 1-week recovery,samples of vastus intermedius were collected at the 5th week. Skeletal muscle cells uhrastructural damage,and nerves,blood vessels structural damage , protein levels of collagen I in depositive collagen fiber, and collagen structural change of skeletal muscle were observed. Results After 4-week repetitive eccentric exercise,skeletal muscle overuse injuries,including Z-band streaming,widened intermyofibrillar spaces,myofibril degradation, aggregated subsarcolemmal mitochondria,fibrosis of nerves and blood vessels,thickened endomysium （especially perimysium）,and increased collagen [ protein levels in 1 RG appeared. Needle puncture weakened the overuse injuries in 1 RAG as compared to that in 1 RG （P 〈 0.01）,including decreased myofibril degradation,restoration of uhrastructural damage in skeletal muscle cells,increase in the numbers of mitochondria,alleviated nerves and blood vessels structural damage,and decreased collagen I protein levels in expanded endomysium,particularly in perimysium. Conclusions Acupuncture could effectively treat eccentric exercise-induced skeletal muscle overuse injuries. The effectiveness of acupuncture seems to be associated with increased numbers of mitochondria in skeletal muscle.