中文摘要：

目的:观察青藤碱(sinomenine,SIN)对大鼠腹腔巨噬细胞吞噬能力的影响,并对其机制进行探讨。方法:大鼠随机分为对照组和青藤碱(20、60、180mg/kg)4组,酵母多糖A腹腔注射诱导急性腹膜炎,提取腹腔巨噬细胞,贴壁法纯化。鸡红细胞吞噬法测定巨噬细胞吞噬指数和吞噬率;分别采用荧光定量realtimeRT-PCR和免疫组织化学方法检测TNF-α、IL-6mRNA和蛋白表达。结果:20mg/kg青藤碱能明显提高巨噬细胞吞噬吞噬指数和吞噬率,明显降低巨噬细胞TNF-α、IL-6的表达;60、180mg/kg组能显著提高巨噬细胞吞噬指数和吞噬率,显著降低巨噬细胞TNF-α、IL-6的表达。结论:青藤碱能显著提高巨噬细胞吞噬能力,减少炎症因子表达。

英文摘要：

Aim:To study the effects of sinomenine(SIN)on phagocytosis of macrophages and its potential mechanism.Method:The rats were divided into four groups randomly(n =8),i.e.one control group and 3 treated groups(SIN20,60 or 180 mg/kg,ig).Acute peritonitis was induced by zymosan A(20mg/kg)injection peritoneally in rats.Phagocytosis function of peritoneal macrophages was measured by chick-en red blood cell phagocytosis assay in vitro.mRNA and protein expression levels of interleukin 6(IL-6)and tumor necrosis factor...