研究玉米储藏过程中霉菌类群与玉米赤霉烯酮毒素(ZEN)的关系。将不同初始水分含量的玉米在30℃,不同相对湿度环境(RH)中储藏28 d。结果表明:低湿环境(RH 75%)木霉数量抑制ZEN含量程度随初始水分升高而大幅增强;中、高湿度环境(RH 84%和92%)木霉抑制ZEN效果增强,受初始水分影响小。RH 92%环境,初始水分12.4%、14.3%时霉菌丰富度高,19.9%时菌群丰富度低。经相关性分析,RH75%玉米霉菌总数、木霉、串珠镰刀菌数和水分含量均与ZEN积累量呈负相关;而RH 92%环境,均呈正相关;RH 84%环境,木霉数量和水分含量与ZEN积累量呈显著正相关(P〈0.05),镰刀菌和霉菌总量与ZEN积累量呈负相关。中低湿度环境,ZEN含量易受生物、环境因素影响;高湿度环境,ZEN含量主要由镰刀菌数控制。
This paper studied the relationship between the mold species and zearalenone production of corn under different storage conditions. The results showed that,in the low humidity environment( RH 75%),the effect that Trichoderma viride suppressing the content of zearalenone was affected by initial moisture content significantly,and it increased rapidly with the initial moisture increasing. The zearalenone was strongly inhibited by Trichoderma viride rather than initial moisture at the medium and high humidity condition( RH 84% and RH 92%). The mold diversity with initial moisture content of 12. 4% and 14. 3% increased,but with 19. 9% initial moisture it was decreased at RH 92%. The number of Fusarium moniliforme and moisture content were negative correlation with the accumulation of zearalenone,but positive under RH 92%. The Trichoderma viride and water component were respectively significant positive correlation with zearalenone( P〈0. 05) at relative humidity of 84%. The total number of mould and Fusarium moniliforme was negative correlation with the zearalenone,respectively. The result showed that zearalenone was strongly influenced by biological and environmental factors at the medium and low humidity condition. Moreover,the zearalenone was strongly influenced by Fusarium moniliforme at high humidity condition.