中文摘要：

本实验将自聚发光分子四苯基乙烯（ TPE）和聚集淬灭分子阿霉素（ DOX）连接为荧光分子复合物（ TPE？DOX conjugate， TD），并通过荧光光谱分析和扫描电镜观察结合的方法，对TD纳米聚集体中TPE和DOX的荧光淬灭机制进行了研究。实验发现，在TD分子内，TPE和DOX之间发生了“能量传递接力”的现象，即TPE通过荧光能量共振转移将能量传递给DOX，导致自身荧光发射淬灭；DOX由于TD纳米聚集体的形成，发生了聚集淬灭现象，致使TPE转移的能量和DOX自身的荧光发射均被淬灭，达到一个荧光双淬灭的效果。研究中采用荧光分析与电镜观察结合的方法，阐述了将两种具有相反发光性质的荧光分子在纳米尺度的荧光淬灭机理，为纳米尺度上的分子内荧光淬灭研究提供了理论依据。

英文摘要：

In this paper, tetraphenylethene （ TPE） and doxorubicin （ DOX） were conjugated together, forming a complex fluorescent molecule （ TPE？DOX conjugate, TD） . By evaluating the fluorescence spectra and scanning electron microscopy results, fluorescence quenching mechanism of TPE and DOX in TD nanoaggregates was studied. The experiment results indicated that between TPE and DOX, a “energy transfer relay” phenomenon occurred, i. e. , TPE transferred its emissive energy to DOX through fluorescence resonance energy transfer （ FRET） , leading to its fluorescence emission quenched, and the fluorescence of DOX was also quenched by means of aggregation cause quench, and thus resulting in a “double quenched” fluorescence behavior. By combining scan electron microscopy observation and fluorescence spectra analysis, this paper provided a novel theoretical mechanism for studying nanoscaled fluorescence quenching behaviors.