中文摘要：

目的：研究虫草素对A549细胞增殖、凋亡及核转录因子NF-κB的影响,并探讨其诱导凋亡的机制。方法：采用MTS法检测不同浓度虫草素对A549细胞的增殖抑制作用,并计算24 h的IC50;用Hoechst 33258染色法及流式细胞术检测24 h不同浓度虫草素诱导A549细胞凋亡的情况;Western blot法检测虫草素对A549细胞核内NF-κB p65及凋亡相关蛋白BAX、BCL-2、cleaved Caspase-3表达的影响。结果：虫草素能明显抑制A549细胞增殖,促进其凋亡;随着虫草素浓度的升高,BAX和cleaved Caspase-3的表达上调,而BCL-2表达下调;Western blot检测结果显示,虫草素能抑制A549细胞质中的NF-κB p65向核内转位。以上作用均呈现量效关系。结论：虫草素能抑制A549细胞增殖并促进其凋亡,机制可能与抑制NF-κB通路相关。

英文摘要：

Objective： To investigate the effects of Cordycepin on proliferation and apoptosis of A549 cells,and its possible mechanism of action. Methods： Human lung cancer cell line A549 cells were treated with different concentrations of Cordycepin for 24 h. MTS assay was used to detect cell proliferation. Hoechst 33258 staining and Annexin V / PI flow cytometry were used to determine the apoptosis of A549 cells. The protein expression of nuclear factor-κB p65（ NF-κB p65）,BAX,BCL-2 and cleaved Caspase-3 was determined by Western blot. Results： In a dose dependent way,Cordycepin inhibited the proliferation and promoted the apoptosis of A549 cells,increased the expression of BAX and cleaved Caspase-3,while decreased the expression of BCL-2. The Western blot results showed,Cordycepin dose-dependently inhibited the entry of NF-κB p65 to nuclear in A59 cells. Conclusion： Cordycepin can inhibit the proliferation and induct the apoptosis of A549 cells,the mechanism of action is achieved by inhibiting the NF-κB pathway.