中文摘要：

目的探讨wnt3对小鼠肝前体细胞（14-19）发生上皮-间质转化的影响。方法分别将空载体Ad-GFP和表达wnt3的腺病毒Ad-GFP-wnt3转入小鼠肝前体细胞中，显微镜下观察细胞形态变化。细胞划痕实验和细胞迁移实验观察14-19细胞迁移能力。实时荧光定量PCR和蛋白质印迹分析分别检测14-19细胞中上皮标记物和间质标记物的表达改变。结果显微镜下可见高表达wnt3的14-19细胞由不规则多边形变为长梭形。细胞划痕实验和细胞迁移实验结果显示，高表达wnt3的14-19细胞迁移能力明显提高，与空载体转染细胞相比差异具有统计学意义（P〈0．01）。实时荧光定量PCR和蛋白质印迹结果显示，问质标记物N-cadherin、vimentin和twistl的mRNA和蛋白水平表达上调，相反上皮标记物E-cadherin的mRNA水平和蛋白水平表达下调，与空载体转染细胞相比差异具有统计学意义（P〈0．01）。结论Wnt3能够促使小鼠肝前体细胞发生上皮-间质转化，提示其可能参与了肝纤维化的发展进程。

英文摘要：

Objective To investigate the impact of wnt3 upon epithelial-mesenchymal transition in mouse hepatic progenitor cells （14-19 cells）. Methods Ad-GFP-wnt3 virus and blank Ad-GFP were transfected into 14-19 cells; cell morphology was observed under microscope. The migration ability of 14-19 cells was examined by wound-healing assay and transwell assay. The expression of epithelial and mesenchymal markers in 14-19 cells was detected by real-time PCR and Western blotting analysis. Results Microscopic observation found that 14-19 cells changed from an epithelial characteristic shape into a spindle-like shape. Wound-healing assay and Transwell assay showed that the migration ability of 14-19 cells highly expressing wnt3 was significantly enhanced compared with the blank control group （P~0. 01）. Real-time PCR and Western blotting analysis showed that mesenchymal markers N-cadherin, vimentin and twistl were significantly increased at both mRNA and protein levels in Ad-GFP-wnt3 virus group than the blank control group, while the epithelial marker E-cadherin was significantly decreased at mRNA and protein levels（P~0.01）. Conclusion Wnt3 can induce epithelial-mesenchymal transition in mouse hepatic progenitor cells, indicating that wnt3 may take part in the progress of hepatic fibrosis.