中文摘要：

目的：探讨不同浓度的谷红注射液对乳鼠海马神经元因缺氧缺糖（OGD）所造成损伤的保护作用。方法：培养原代乳鼠海马神经元并用神经元特异性烯醇化酶（NSE）免疫组化染色法鉴定。将培养成熟的细胞随机分成6组：正常组、OGD模型组、尼莫地平组（200g/m L）、谷红注射液低、中、高剂量（30、60、90g/m L）组,除正常组之外,余组都加药并进行OGD处理,造模后检测各组乳鼠海马神经元细胞中丙二醛（MDA）、一氧化氮（NO）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-PX）表达活性及细胞上清液乳酸脱氢酶（LDH）外漏量的变化情况,采用酶联免疫吸附法检测各组细胞上清液中白细胞介素-6（IL-6）以及单核细胞趋化蛋白-1（MCP-1）含量,采用流式细胞术检测谷红注射液对各组海马神经元细胞凋亡的影响。结果：鉴定表明,海马神经元纯度〉90%,可用于实验。OGD使原代培养的海马神经元受损,与OGD模型组相比,谷红注射液高、中、低剂量组可以有效减轻缺氧造成的损伤,能够有效提高SOD与GSH-PX活性,减轻MDA表达,减少LDH外漏以及NO含量,减弱MCP-1与IL-6表达,抑制海马神经元凋亡（P〈0.05,P〈0.01）。结论：谷红注射液可以减轻新生乳鼠海马神经元OGD损伤,其保护机制可能与增强细胞抗氧化应激能力、减轻炎性反应与抗细胞凋亡有关。

英文摘要：

Objective：To study the protective effects of Guhong Injection on neonatal rat primary cultured hippocampal neurons injured by oxygen-glucose deprivation.Methods：The neonatal rat primary cultured hippocampal neurons were identified by the neuron specific enolase nse（NSE） immunohistochemical staining method.The matured hippocampal neurons were randomly divided into six groups：control group,OGD model group,positive control group（Nimodipine,200mg/mL）,Guhong Injection groups of different dosage（30,60,90g/mL）.Except for the control group,other groups were established the oxygen-glucose deprivation model.The intracelluar content of malondiuldehyde（MDA）,the nitric oxide（NO）,the activities of superoxide dismutase（SOD）,the glutathine peroxidase（GSH-PX） and the leakage of lactate dehydrogenase（LDH） were measured by assay kits after modeling in each group.The levels of interleukins-6（IL-6） and monocyte chemoattractant protein-1（MCP-1） in the supernatant of cells were detected by enzyme-linked immunosorbent（ELISA） method.The effects of Guhong Injection on cell apoptosis was detected by flow cytometry.Results：The results of NSE identification proved that the purity of cultured hippocampal neuron reached more than 90%.Compared with model group,Guhong Injection groups especially for the Guhong Injection high-dose group remarkably inhibited the release of LDH,enhanced the activities of SOD and GSH-PX and decreased the content of MDA and NO.Also,the levels of IL-6 and MCP-1 in hippocampal neurons were down-regulated by the administration of Guhong Injection（P〈0.05,P0.01）.Additionally,Guhong Injection reversed the apoptosis induced by oxygenglucose deprivation.Conclusion：Guhong Injection exhibites its protective effects against the damage of hippocampal neurons injured by oxygen-glucose deprivation,for which mechanism underlying is likely related with enhancing the ability of clearing oxidative stress,inhibiting the production of inflammatory factors and reducing the cell