中文摘要：

目的克隆大鼠电压依赖性阴离子通道1（VDAC1）基因,并应用生物信息学方法进行分析。方法以提取的大鼠心肌组织总RNA为模板,RT-PCR扩增获得VDAC1基因片段,连接到pMD19-T克隆载体并测序鉴定。应用生物信息学方法分析VDAC1基因的序列同源性与结构特征。结果成功克隆了大鼠VDAC1基因,与人、牛、小鼠、兔和猪的VDAC1基因具有高度同源性,同源性分别为90.0%、90.6%、96.0%、91.1%和91.0%。VDAC1在体外哺乳动物网状细胞中的半寿期为30h,无卷曲螺旋结构,但有6个亲水区,在225～247aa具有真核细胞线粒体外膜孔道蛋白家族特征性模序结构。结论成功克隆了大鼠VDAC1基因,并应用生物信息学技术进行序列分析,为进一步研究其生物学功能及其在2型糖尿病发病机制中的作用提供了依据和线索。

英文摘要：

Objective Voltage-dependent anion channel 1（VDAC1）was involved in type 2 diabetes in the process of oxidative stress,possibly through changing the balance of oxidation system and antioxidant system in cells.Rat is commonly used in the shudy of diabetes.The aim of this study is to clone the rat target gene VDAC1,and analyze the gene by bioinformatics methods,with a view to provide information for further study on the biological function of VDAC1 and the possible role in the pathogenesis of diabetes.Methods The DNA fragment of VDAC1 gene was amplified by reverse transcription polymerase chain raction（RT-PCR）with mRNA from rat myocardium tissue as the template,and ligated into pMD19-T cloning vector.After sequencing,the correct DNA fragment was identified.Bioinformatics technique was used to analyze the gene sequence homology and structural feature of VDAC1.Results Rat VDAC1 gene was cloned successfully.The rat VDAC1 gene was highly homologous to that of human（NM_003374）,cattle（NM_174485）,mouse（NM_011694）,rabbit（NM_001082075）and pig（NM_213960）in GenBank with the homology of 90.0%,90.6%,96.0%,91.1% and 91.0%,respectively.Bioinformatics analysis showed the half-life of VDAC1 was 30 hours（mammalian reticulocytes,in vitro）.It was also shown that VDAC1 had no spiral coil region,but had six hydrophilic regions.In addition,the motif structure prediction showed VDAC1 had an eukaryotic mitochondrial porin signature in 225-247aa.Conclusion Rat VDAC1 gene has been cloned successfully and analyzed by bioinformatics technique.It provides some evidences and clues for the further study on biological functions of rat VDAC1 gene,and on its role in type 2 diabetes mellitus（T2MD）.