中文摘要：

基于光致电子转移原理（Photoinduced Electron Transfer,Pe T）,建立了以5-（4-马来酰亚胺基苯基）-10,15,20-三（4-甲基苯基）卟啉（MPTMPP）为荧光增强型探针,对巯基蛋白质特异性进行检测。以吡咯、对硝基苯甲醛、对甲基苯甲醛等为主要原料合成了目标化合物MPTMPP,并通过核磁、IR光谱等对MPTMPP进行表征;探究了MPTMPP作为荧光探针对含有巯基的牛血清白蛋白（BSA）的特异性检测效果,该探针对BSA检测的线性范围为0~2.0×10^-8mol/L,线性方程为ΔF=68.588＋36.524[BSA]（10^-8mol/L）,相关系数为0.996 9,检出限为0.037 nmol/L。

英文摘要：

5-（ 4-Maleimidophenyl）-10,15,20-tri-（ 4-methyphenyl） porphrin（ MPTMPP） is synthesized by using pyrrole,4-nitrobenzaldehyde and 4-methylbenzaldehyde as the main raw materials,which is characterized by NMR,FTIR,etc.. It is then used as a fluorescene-enhanced probe for specific detection of thiol-proteins based on the principle of photoinduced electron transfer（ PET）. The specific detection effect of BSA is performed. The linear range for detection of BSA is 0- 2. 0 × 10^-8mol / L with linear equation as ΔF = 68. 588 ＋ 36. 524[BSA ]（ 10^-8mol / L） and correlation coefficient as 0. 996 9. The detection limit for BSA is 0. 037 nmol / L.