中文摘要：

用荧光法对光诱导野生型肌红蛋白（Mb）和突变体（D44K）去氧的过程进行对照研究。发现430nm是研究Mb（WT）和Mb（D44K）光照去氧的最佳激发波长。430nm激发时,Mb（D44K）在597.9nm和628.8nm处出现两个荧光发射峰,不同于Mb（WT）仅在597nm处出现一个荧光发射峰。经研究证明,628.8nm处荧光峰是Mb3＋-H2O型中的H2O峰。光照也使此峰的荧光强度下降,但比去氧的速率慢。研究发现,597nm处Mb（D44K）的荧光效率比Mb（WT）的荧光效率低。传能实验表明Mb表面44位氨基酸由天冬氨酸突变为赖氨酸后,不影响Mb（D44K）中色氨酸和酪氨酸残基传递给铁卟啉的荧光效率,但使Mb（D44K）中色氨酸和酪氨酸残基的荧光效率变高。

英文摘要：

The photo-induced deoxygenization of myoglobin and it＇s mutant, Mb （D44K） was studial by the established fluorescence spectroscopic. Mb （IM4K） has 628.8 nm peak besides 597.9 nm which assigns to H2O binging to the Mb^3＋. The illumination not only causes the release of oxygen from the opening of the heme-pockets, but also causes the release of H2O. Its fluorescence efficiency at 597 nm is lower than that of Mb（WT） （wildtype） no matter what the exciting wavelength is 430 nm or 409 nm. The third difference is that the energy transfer experiment shows that the mutation （Lys44 displacing Aspd4） does not influence the efficiency between tryptophan, tyrosine and Fe-porphyrin, but makes the efficiency of intrinsic fluorescence of Mb higher. In addition, the result it implies that the maximum absorption is not always the best exciting wavelength through the fact that 430 nm is better for the release of oxygen from the opening of the heme-pockets.