中文摘要：

以苏云金杆菌和白僵菌为有效成分制成混配菌剂，采用高效小型粉碎机将筛选的助剂及原药混匀，在测定助剂对微生物活体的生物活性、制剂性能影响的基础上，进行混配菌剂助剂的筛选。通过生物测定筛选获得最佳混配比例，并将其加工成可湿性粉剂。结果表明：苏云金杆菌库斯塔克亚种和球孢白僵菌最佳混配质量比为3：1，润湿分散剂为1％MorwetEFW、3％SDS、9％MorwetD425、9％TERSPERSE2020；紫外保护剂为0．3％黄原胶，以凹凸棒土为载体补足100％。以此配方制备的混配菌剂的质量悬浮率72．73％，孢子悬浮率75．13％，润湿时间〈1min，平均粒度为13．76μm。苏云金杆菌和白僵菌可湿性粉剂中各指标符合商品制剂的标准，对黄褐天幕毛虫毒力分别高于苏云金杆菌和白僵菌可湿性粉剂单剂，是一种具有高杀虫活性、持效期长、环境友好型绿色农药。

英文摘要：

The optimal adjuncts tested were screened based on the effects of adjunct on microbial activities and formulation properties. The wettable powder of Bacillus thuringiensis and Beauveria bassiana was prepared in accordance with formulation standards using a muller mixer to blend B. thuringiensis, B. bassiana and adjuncts. The results showed that the optimal ratio of B. thuringiensis and B. bassiana as a wettable powder in accordance with formulation standards was 3： 1, including 1% Morwet EFW, 3% SDS, 9% Morwet D425, 9% TERSPERSE 2020, 0.3% xanthan and carrier to 100%. The suspension percentage, conidia germination and average particle size were 72.73% , 75.13% and 13.76 txm, respectively, and the wetting time for the formulated material was shorter than 1 min. Every parameter of the wettable powder formulations of B. thuringiensis and B. bassiana was in accordance with formulation standards. The wettable power formulations containing both B. thuringiensis and B. bassiana had higher insecticidal efficacy to Malacosoma neustria testacea than wettable powder formulations containing B. thuringiensis or B. bassiana alone. Therefore, the wettable powder of B. thuringiensis and B. bassiana is environment-friendly pesticide with high insecticidal activity and long persistence.