中文摘要：

分析T细胞信号传导分子TCRξ链基因在B细胞-急性淋巴细胞白血病（B—ALL）病人外周血中的表达水平。方法：采用SYBR Green Ⅰ荧光定量PCR和相对定量检测15例初发B—ALIL、5例完全缓解期（CR）患者和30例正常人外周血的单个核细胞的TCRξ链表达情况，以β2微球蛋白基因（β2M）作为内参，根据相对定量公式（2^△△Ct）分别计算B—ALL初发患者、CR期患者与正常人TCRξ链表达差异倍数等。结果：根据相对定量公式分析显示：与正常人相比，B—ALL初发患者外周血TCRξ链表达存在3种情况，即不表达（20％）、表达下降（53％）和表达上升（27％）。TCRξ链表达水平在所有初发B—ALL病人均低于CR期病人。结论：本研究首先报道B-ALL病人中TCRξ链表达水平的变化特点，TCRξ链基因表达水平以降低为主，可能与T细胞免疫缺陷有一定关系。

英文摘要：

Objective ： To analyze the expression level of signal transduction factor T lymphocyte-TCRξ chain gene in patients with B-acute lymphoblastic leukemia （B-ALL）. Methods：Real-Time PCR with SYBR Green Ⅰ technique was used for detecting TCRξ chain expression level in peripheral blood mononuclear cells from 15 patients with untreated de novo B-ALL, 5 patients with B-ALL completely remission （CR） and 30 normal individuals. β2 - microglobulin gene （ 132 M） was used as an endogenous reference. Relative changes in TCRξ chain expression levels was used by the 2^△△Ct method among patients with B-ALL, CR patients, and normal individuals. Results： The expression level of TCRξ chain in patients with B-ALL could be divided into absent expression （ 20% ）, down expression （53%） and over expression （27%） groups. The expression level of TCRξ chain in all de novo B-ALL patients was lower than that in CR patients. Conclusions： It is to our knowledge, the first description in the expression feature of TCRξ chain in B-ALL. The expression level of TCRξ chain was down expression in the most of B-ALL patients, and might relate to cellular immuneodeficiency.