中文摘要：

目的：建立实时定量PCR方法检测TCRξ链表达水平的方法，了解慢性粒细胞白血病（CML）外周血TCRξ链表达水平。方法：采用SYBRGreenI荧光定量PCR，相对定量检测30例CML患者和30例正常人外周血的单个核细胞的TCRξ链表达情况，以β2微球蛋白基因（β2M）作为内参，根据相对定量公式：2^-△△C计算CML病人与正常人TCRξ链表达差异倍数。结果：成功建立SYBRGreenI荧光实时定量PCR检测TcR（链表达检测技术。18例CML患者TCRξ链出现表达低于正常人，而12例CML患者TCRξ链出现表达高于正常人。结论：CML病人中TCRξ链表达水平可分为表达下调（60％）和表达上调（40％）2组，提示部分CML病人的细胞免疫缺陷可能与其TCRξ链表达下调有关。

英文摘要：

AIM ： To establish a real - time PCR technique for detection and quantification of TCR ξ chain expression and to investigate TCR ξ chain expression level in patients with chronic myeloid leukemia （CML）. METHODS： Real-time PCR with SYBR Green I technology was used for detecting TCR ξ chain expression level in peripheral blood mononuclear cells from 30 patients with CML and 30 normal individuals. β2 - microglobulin gene （ β2 M） was used as an endogenous reference. Relative changes in TCR ξ chain expression level were used by the 2^-△△Ct, method between patients with CML and normal individuals. RESULTS： The SYBR Green I real - time technique for quantitative detection of TCR ξ chain expression levels was established successfully. The expression level of TCR ξ chain in 18 patients with CML was reduced. However, the TCR ξ chain expressed increased in 12 patients with CML. CONCLUSION： The TCR ξ chain expression level is divided into down expression （60%） and over expression （40%） groups, and the down expression of TCR ξ chain might related to cellular immunodeficiency in most of CML patients.