中文摘要：

抓紧域丝氨酸朊酶(cSPs ) ，他们的相当或相同的事物(SPH ) 在是细胞外的发信号的串联的必要部件的各种各样的生物进程起一个重要作用，特别在无脊椎动物的天生的有免疫力的回答。这里，从游泳螃蟹 Portunus trituberculatus 的 PtcSP 和 PtSPH 的多型性被调查与抵抗 / 危险性探索他们的协会到 Vibrio alginolyticus。多态的 loci 用 Clustal X 被识别，并且与社会科学统计套装软体描绘了在抵抗、易受影响的股票之间的遗传型和等位基因频率的 16.0 软件，然后意义被 ² 测试决定。109 和 77 单个核苷酸多型性(SNP ) 的一个总数分别地在 PtcSP 和 PtSPH 的 genomic 碎片被识别。尤其是，将近， PtSPH 多型性的一半在非编码的 exon 被发现 1。调查的十四 SNP 显著地与危险性 / 抵抗被联系到 V。alginolyticus (P < 0.05 ) 。在他们之中，八 SNP 在 introns，和 introns 被观察同义，四非同义的 SNP 和一 ins-del 在编码 exons 被发现。另外，五次简单顺序重复(SSR ) 在 intron 被检测 3 PtcSP。尽管没有等位基因频率的统计上重要的差别， SSR 根据在抵抗、易受影响的股票之间的重复数字显示出不同多态的等位基因。在进一步的确认以后，这里调查的多型性可能被使用选择 P 的潜在的分子的标记。有到 V 的抵抗的 trituberculatus。alginolyticus。

英文摘要：

Clip domain serine proteases （cSPs） and their homologs （SPHs） play an important role in various biological processes that are essential components of extracellular signaling cascades, especially in the innate immune responses of invertebrates. Here, polymorphisms of PtcSP and PtSPH from the swimming crab Portunus tritubereulatus were investigated to explore their association with resistance/ susceptibility to Vibrio alginolyticus. Polymorphic loci were identified using Clustal X, and characterized with SPSS 16.0 software, and then the significance of genotype and allele frequencies between resistant and susceptible stocks was determined by a Zz test. A total of 109 and 77 single nucleotide polymorphisms （SNPs） were identified in the genomic fragments of PtcSP and PtSPH, respectively. Notably, nearly half of PtSPH polymorphisms were found in the non-coding exon 1. Fourteen SNPs investigated were significantly associated with susceptibility/resistance to I1. alginolyticus （P〈0.05）. Among them, eight SNPs were observed in introns, and one synonymous, four non-synonymous SNPs and one ins-del were found in coding exons. In addition, five simple sequence repeats （SSRs） were detected in intron 3 of PtcSP. Although there was no statistically significant difference of allele frequencies, the SSRs showed different polymorphic alleles on the basis of the repeat number between resistant and susceptible stocks. After fiarther validation, polymorphisms investigated here might be applied to select potential molecular markers ofP. trituberculatus with resistance to I1. alginolyticus.