中文摘要：

目的观察锌指转录因子snail在耐顺铂（eisplatin，CDDP）的人小细胞肺癌（smallcelllungcancer，SCLC）H446／CDDP细胞中的表达情况，探讨snail表达在人SCLC顺铂耐药中的作用。方法培养H446细胞系和H446／CDDP细胞系，采用MTT法检测H446／CDDP的耐药指数；分别提取人SCLC顺铂耐药细胞H446／CDDP及其亲代细胞H446的mRNA及总蛋白，应用sybgreen实时荧光liT—PCR、Westernblot方法检测snailmRNA及蛋白表达水平。结果H446／CDDP细胞snail蛋白及mRNA表达水平较H446细胞显著性升高（P〈O．05）。结论SCLC的顺铂耐药性可能与snail表达水平增高有关。

英文摘要：

Objective To observe the expression of zinc finger transcription factor snail in human cis- platin （CDDP）-resistant small-cell lung cancer （SCLC） cell line H446/CDDP, and to study the role of snail in cisplatin resistance of human SCLC. Methods H446 and H446/CDDP cell lines were cultured, and cisplatin resistance index was measured by MTr assay. The mRNA and protein expression levels of snail in the H446 and H446/CDDP cells were detected by real-time RT-PCR and Western blotting. Results The mRNA and protein expression levels of snail in the H446/CDDP cells were significantly higher than those in the H446 cells （P 〈 0. 05 ）. Conclusion The CDDP resistance of human SCLC may be related to the high expression level of snail.