中文摘要：

酵母单杂交系统是由酵母双杂交系统衍生来的研究DNA与蛋白质之间相互作用的新型系统。该文系统阐述了单杂交系统的基本原理和技术路线,详细综述了其在植物基因工程各个领域的应用研究进展,即克隆抗渗透胁迫类转录因子基因,确定已知DNA与蛋白质之间的相互作用,定位已证实的具有相互作用的DNA结合结构域以及验证转录激活作用;并分析了当前该系统在植物基因工程研究中存在的问题,进而结合自己的研究对解决问题的途径进行了探讨。

英文摘要：

The yeast one hybrid system is the new type system deriving from yeast two hybrid system to study the interactions between DNA and protein. This paper briefly introduces the principle and technical line of this system, and particularly summarizes the progress of this system in plant genetic engineering field, such as cloning the transcription factor genes of osmosis resistance type, of embryogenesis type, and of disease resistance type; confirming the interaction between known DNA and protein; making sure the localization of the confirmed DNA binding domain ; moreover validating the transcriptional activation activity. Furthermore, the authors analyzed the problems existing in this system on the research of plant genetic engineering, as well as forecasted the development prospect according to the research.