中文摘要：

目的建立人血管内皮细胞金属硫蛋白2A（MT2A）上调表达细胞模型。方法构建重组质粒pEGFP-N1-MT2A,转染至人脐静脉内皮细胞（HUVECs）,荧光显微镜观察转染效果,RT-PCR及蛋白印迹法测定细胞MT2A表达水平。结果测序证实pEGFP-N1-MT2A重组质粒构建正确,转染至HUVECs后,通过RTPCR蛋白印迹实验证实细胞MT2A表达水平升高。结论成功构建了重组质粒pEGFP-N1-MT2A,转染至HUVECs可使MT2A表达水平升高。

英文摘要：

Objective To establish a kind of HUVECs cell model which can up-regulate the expression of MT2A. Methods The recombinant plasmid pEGFP-N1-MT2A was constructed and transfected to HUVECs. The effective of transfection was confirmed by observing with fluorescence microscope. RT-PCR and Westernblot test were used for determining the expression of MT2A in HUVECs. Results The recombinant plasmid pEGFP-N1 MT2A was confirmed by sequencing,green-stain cells were observed with fluorescence microscope after the plasmid was transfected to HUVECs. The expression of MT2A was up-regulated by transfecting pEGFP-N1-MT2A to HUVECs. Conclusion Recombinant plasmid pEGFP-N1 MT2A is constructed successfully; expression of MT2A can be up-regulated by transfecting the recombinant plasmid to HUVECs.