中文摘要：

目的：探讨γ-干扰素（IFN-γ）诱导小鼠系膜细胞内脂质沉积的可能机制。方法：常规培养的小鼠系膜细胞（MMC）分为正常对照组、刺激组、刺激＋空质粒组（sh-HMGB1）和刺激＋质粒组（sh-SREBP-1）;油红O染色观察细胞内脂质沉积;RT-PCR检测HMGB1、SREBP-1和脂肪酸合成酶（FAS）mRNA表达;Wesern blot检测蛋白表达。结果：油红O检测显示IFN-γ刺激组MMC细胞中出现明显脂滴;IFN-γ刺激能够上调HMGB、SREBP-1和FASmRNA及蛋白表达;沉默HMGB1能够降低IFN-γ诱导的SREBP-1和FAS上调,并减少细胞内脂质沉积;沉默SREBP-1能够减少HMGB诱导的MMC细胞内脂质沉积。结论：IFN-γ可能通过上调HMGB/SREBP-1/FAS的表达促进小鼠系膜细胞内脂滴沉积。

英文摘要：

Objective： To explore the possible mechanism of lipid deposition induced by interferon-γ（IFN-γ）.Methods： The mouse mesangial cells（MMC） were randomly divided into control group,stimulation group,stimulation＋control vector group（sh-HMGB1） and stimulation＋specific sh-vector group（sh-SREBP-1）. RTPCR was used to detect the expression of HMGB1,SREBP-1 and fatty acid synthetase（FAS） mRAN;the protein expression was determined by Western blot.Results： The Oil Red O staining revealed that the mouse mesangial cells showed significant lipid droplet in IFN-γ group.IFN-γ up-regulated the expression of HMGB1,SREBP-1,FAS mRNA and protein time-dependently;Transfection of MMC with HMGB1 siRNA resulted in the suppression of SREBP-1,FAS protein levels induced by IFN-γ,following with decrease of lipid deposition.Stimulation with HMGB1 markedly induced expression of SREBP-1,FAS expression and peaked at 8 h,decreased at 12 h compared with that at 8 h.Sh-SREBP-1 decreased the lipid deposition induced by HMGB1 in MMC.Conclusion： IFN-γ might induce lipid deposition in mouse mesangial cells partly by up-regulating the expression of HMGB1/SREBP-1/FAS.