中文摘要：

该实验以小鼠系膜细胞MMC为研究对象，以重组HMGBl为刺激物，通过检测细胞周期的变化及细胞中PCNA、CyclinD1、CDK4和p16的表达水平．初步探讨HMGB1对系膜细胞的细胞周期及其相关调控因子的影响。选取小鼠系膜细胞MMC为研究对象，随机分为对照组及0．05mg／LHMGB1刺激组，经流式细胞术检测发现HMGBl能够上调小鼠系膜细胞中s期细胞所占比例：免疫细胞化学检测显示，PCNA蛋白在小鼠系膜细胞中的表达上调：通过R]乙PcR技术及Western blot技术检测到小鼠系膜细胞中CyclinD1mRNA和蛋白以及CDK4蛋白的高表达情况，而p16蛋白的表达呈时间依赖性降低。由此可见，HMGB1可能是通过上调CyclinD1／CDK4的表达，并下调p16的表达，促进细胞从G0／G1期进入s期，介导了小鼠系膜细胞的异常增殖，可能是HMGB1参与狼疮性肾炎发病的可能机制之一。

英文摘要：

At this study we detected the distribution of cell cycle and the expression changes of PCNA, CyclinD1, CDK4 and p16 in MMC. We also analyze the relationship among them in order to explore the possible effect of HMGB 1 on the generation of MMC. MMC were obtained from the Department of Medical Pathology of Hebei Medical University. MMC selected for the study were randomly divided into control group and HMGBI stimulation group （0.05 nag/L）. The cells were collected after 4, 8, 12 h. The changes in cells cycle distribution were detected by flow cytometry. Immunocytochemical stain were used to detect the over expression of PCNA protein in MMC; the level of CyclinD1 mRNA were detected by RT-PCR; Western blot detected that HMGB1 up-regulated the levels of CDK4 protein and the CyclinD1 protein in MMC, decreased the expression of p16 protein in MMC. We can see that： HMGB1 could induce mouse mesangial cells proliferation and promote the transition of cell cycle from G1 stage to S stage by up-regulating CyclinD1/CDK4/pl6 pathway, which might be an effect mechanism of HMGB 1 in lupus nephritis pathogenesis.