中文摘要：

以氨气扩散法为基础,以再生丝蛋白（RSF）作为可溶性有机物对磷酸钙的生成实施调控.采用透射电镜包括选区电子衍射、扫描电镜、X-射线衍射及红外光谱等多种技术手段对所生成磷酸钙的形貌和晶型进行观察和检测.通过改变矿化时间、RSF浓度以及钙/磷离子浓度等,能够在特定的条件下可控获得具有相应形貌即球形颗粒状的无定形磷酸钙（ACP）或粘连片状的羟基磷灰石（HAP）.大量的实验显示出其基本规律为,在较高钙/磷离子浓度的母液中,诱导形成ACP所需的RSF浓度也较高;而在其它相同的情况下,RSF的分子量越高,其调控产生ACP所需的浓度随之降低.同时,RSF不仅对ACP的生成具有诱导效应,而且还能起到对ACP的稳定作用.相对稳定的ACP/RSF杂化物对骨修复材料的制备而言具有非常重要的意义.

英文摘要：

Amorphous calcium phosphate（ ACP） has excellent biocompatibility,osteoconduction and biodegradability. However,as it can be transformed easily to a more thermal stable phase,i. e. HAP,ACP shows poor stability in solution. As a soluble and biocompatible additive,regenerated silk fibroin（RSF） can mediate the growth of inorganic crystals. If the RSF is capable of inducing and stabilizing of the ACP,it will be desirable to expand its application to the field of bone repair. The pH value of the mineralization solution increased slowly when the ammonia gas diffused into the solution,leading to the precipitation of calcium phosphate. The morphology and polymorph of calcium phosphates were characterized by SEM,TEM,SAED,XRD and FTIR. Generally,the spherical particles were ACP and the aggregated sheets were HAP in our work.The results also showed that ACP could be obtained by adjusting the mineralization time,the weight concentration of RSF,the molar concentrations of calcium ion and phosphate ion. When the RSF and Ca2 ＋/PO43-concentrations were fixed to 0. 5% and 2 mmol /L,respectively,only ACP could be obtained after 1 h;however,both ACP and HAP could be produced when the duration of mineralization was increased to 3 h.Moreover,all the ACP would be transformed to HAP when the time was increased to 24 h. On the other side,when the Ca2 ＋/PO43-concentrations increased,a higher RSF concentration would be needed to induce the growth of ACP. For example,ACP could be produced under a certain condition that the mineralization time and the RSF concentration were 1 h and 1% respectively,supplemental with 3 mmol /L Ca2 ＋/PO43-concentrations,but ACP couldn’t be obtained at any concentration of RSF when the ion concentration was reached to 5 mmol /L. Furthermore,it was found that RSF with higher molecular weight could induce the growth of ACP in a lower concentration under the same condition. The stable ACP /RSF hybrid may have the great potential in the bone repairing.