欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
位置：立项数据库 > 立项详情页
中文摘要：
非生理活性蛋白质以其无关键氨基酸位点的特性，已成为高分子科学与蛋白质科学交叉研究的很好对象，而规模化的量产，更使其具有成为带特殊应用性能的生物材料。本项目拟在对动物丝蛋白的构象、结构及性能之间关系研究中取得的一系列创新性成果的基础上，通过以下工作来阐明非生理活性蛋白及其肽段在组装过程中的驱动力和各种影响因素，并利用分子（链）之间的各类相互作用，在调控蛋白质（多肽）多层次有序聚集的过程中，赋予其不同性状聚集体各种功能1、具有特征链结构及分子量的丝蛋白（多肽）的制备和聚氨基酸的可控合成，了解影响其差异的各种内外因素；2.对丝蛋白等多肽链在溶液中的行为进行探索，掌握控制蛋白质构象及其先期形成"结构域"的有效方法；3.研究蛋白质（多肽）"结构域"之间的相互影响，由此对进一步形成的"宏观"有序结构在不同层次上进行调控；4、从性状和有序折叠特征等多方面对各种蛋白质（多肽）的聚集体实施功能化。
英文摘要：
silk fibroin；peptide；assembly；regulation；functionalization
结论摘要：
本项目严格按照计划任务书中的工作要点执行且有所拓展，取得了丰硕的成果。项目以非生理活性的天然动物丝/丝蛋白和合成聚氨基酸包括其多肽片段为主要研究对象，通过不同环境下力学性能测试、流变学研究、常规谱学及同步辐射谱学分析等传统和先进研究方法并重的策略，进一步深入地理解了其结构与性能之间关系；在此基础上，不仅实现了具有特征链结构和分子量（分布）的丝蛋白/多肽制备和聚氨基酸的可控合成，而且详尽地考察了其在不同溶液中的构象及转变、自组装特征和聚集态结构等性质；在充分认识到特定氨基酸序列先期折叠而成的“结构域”对蛋白质/多肽/聚氨基酸有序聚集重要作用的前提下，探索了丝蛋白和两亲性多肽/聚氨基酸在组装过程中的驱动力和多种影响因素，利用分子（链）之间的各类相互作用，在调控丝蛋白（多肽/聚氨基酸）多层次有序聚集的过程中，赋予不同性状聚集体的多种功能；同时，也在一定程度上实现了蛋白质/多肽/聚氨基酸对多种无机物形成过程和性状的调控，并使此类有机/无机杂化物具备了特殊的理化性能。
成果综合统计
期刊论文
会议论文
专利
获奖
著作
期刊论文
Poly(styrene-co-acrylic acid) core and silver nanoparticle/silica shell composite microspheres as hi
Conformation Transition of Bombyx mori Silk Protein Monitored by Time-Dependent Fourier Transform In
Amphiphilic Polypeptides as a Bifunctional Template in the Mineralization of Calcium Carbonate at th
Precisely designed rattle-type mTiO(2)@P(NIPAM-co-MBA) microspheres with screening gel network for h
Synthesis of mesoporous magnetic Co-NPs/carbon nanocomposites and their adsorption property for meth
Elucidating the role of free polycationic chains in polycation gene carriers by free chains of polye
Uniform Magnetic Core/Shell Microspheres Functionalized with Ni2+-Iminodiacetic Acid for One Step Pu
Synthesis of poly (gamma-benzyl-L-glutamate) with well-defined terminal structures and its block pol
Surface-Modified Silicon Nanoparticles with Ultrabright Photoluminescence and Single-Exponential Dec
Insight into Polycation Chain Length Affecting Transfection Efficiency by O-Methyl-Free N,N,N-Trimet
Green Synthesis of Silk Fibroin-Silver Nanoparticle Composites with Effective Antibacterial and Biof
The Robust Hydrogel Hierarchically Assembled from a pH Sensitive Peptide Amphiphile Based on Silk Fi
Characterization and assembly investigation of a dodecapeptide hydrolyzed from the crystalline domai
Benzoboroxole-Functionalized Magnetic Core/Shell Microspheres for Highly Specifi c Enrichment of Gly
Determination of phase behaviour in all protein blend materials with multivariate FTIR imaging techn
Multiplexed SERS detection of DNA targets in a sandwich-hybridization assay using SERS-encoded core-
Synthesis of highly surface functionalized monodispersed poly(St/DVB/GMA) nanospheres with soap-free
Investigation on thermally-induced conformation transition of soy protein film with variable-tempera
Microwave-assisted hydrothermal crystallization: an ultrafast route to MSP@mTiO(2) composite microsp
Fe3O4/PVIM-Ni2+ Magnetic Composite Microspheres for Highly Specific Separation of Histidine-Rich Pro
Modulating Materials by Orthogonally Oriented beta-Strands: Composites of Amyloid and Silk Fibroin F
Wet-spinning of regenerated silk fiber from aqueous silk fibroin solutions: Influence of calcium ion
Folate-conjugated poly(N-(2-hydroxypropyl)methacrylamide-co-methacrylic acid) nanohydrogels with pH/
Detecting Trace Melamine in Solution by SERS Using Ag Nanoparticle Coated Poly(styrene-co-acrylic ac
Ultrafast Hydrothermal Synthesis of High Quality Magnetic Core Phenol-Formaldehyde Shell Composite M
Preparation of 3D Fibroin/Chitosan Blend Porous Scaffold for Tissue Engineering Via a Simplified Met
Silk fibroin immobilization on poly(ethylene terephthalate) films: Comparison of two surface modific
Synthesis of hierarchical three-dimensional copper oxide nanostructures through a biomineralization-
Insight into the Structure of Single Antheraea pernyi Silkworm Fibers Using Synchrotron FTIR Microsp
Two-in-One Strategy for Effective Enrichment of Phosphopeptides Using Magnetic Mesoporous gamma-Fe2O
相关项目
邵正中的项目