中文摘要：

为构建缺失不同功能区的猪内源性反转录病毒（PERV）5＇非编码区（5’UTR）克隆，分析5’UTR的转录调控规律，以质粒WZSP-293—5’UTR为模板，应用重组PCR方法分别扩增5’UTR与缺失引物结合区及前导序列（PBS—Leader），即51UTR（5＇长末端重复区）、U3区、R区、U3区中重复序列的51UTR等5种PCR片段，并分别将其克隆至T载体上，挑取阳性克隆，酶切鉴定插入方向，选择正确方向插入的克隆，删序并进行序列分析。结果表明，构建了系列功能区缺失的5种51UTR克隆，分别命名为UTR—PMD、LTR—PMD、UTR—U3-PMD、UTR-R—PMD、UTR—U—PMD以备后用。结论：在PERV5＇UTR的不同功能区中含有一系列可能的转录因子结合位点；中国五指山猪内源性反转录病毒（WZSP—PERV）在293细胞中传代时，不仅U3重复区数目增加，重复亚型也发生了一定的改变。

英文摘要：

To analyze the transcriptional regulation of PERV untranslated region,A series of functional region deletion of PERV 5＇UTR were cloned ,including completed 5＇UTR, 5＇UTR lacking of PBS and Leader （5＇UTR）, 5＇ UTR lacking of U3 region,5＇UTR lacking of R region,5＇UTR devoid of the U3 repeat box. First,we use PCR to amplify the above five fragments,then ligated them to pMD18-T vector,chose the positive clones,restricted them with enzymes to identify the orient of the inserts. The plasmids LTR-PMD,UTR-PMD,UTR-U3-PMD,UTR-RPMD,UTR-U-PMD were constructed for further use,the 5＇UTR sequenced and the potential binding of transcription factors（TFS） predicted by the Kyoto Encyclopedia of Genes and Genomes（KEGG）. It is concluded that there have different potential binding of TFS different functional region of PERV 5＇UTR;When passaging the WZSPPERV in HEK293 cells ,not only the number of U3 repeat box increased but also the subtype changed.