研究Neutrase、Acalase 2.4 L、Papain、Pepsin、Trypsin、Flavourzyme、Acid protease、Protamex与Bromelain 9种蛋白酶水解大米蛋白的进程特性,考察9种蛋白酶水解物的免疫活性。结果表明,胰脏蛋白酶Trypsin可作为制备大米免疫活性肽的最适水解酶,Trypsin酶解物对小鼠巨噬细胞(RAW264.7)的增值指数(stimulating index,SI)为1.597(1 000μg/m L);其酶解物中相对分子质量〈1 000的组分比例最高,达到74.11%。
The hydrolysis processes of rice protein by had been studied 9proteinases including Neutrase,Acalase 2.4 L,Papain,Pepsin,Trypsin,Flavourzyme,Acid protease,Protamex and Bromelain,and the immune activities of 9 protein hydrolysate had been considered.The results showed that Trypsin can be used as the optimum hydrolytic enzymes for the preparation of rice immune active peptide whose value-added index(stimulating index,SI) to mice macrophage(RAW264.7) is 1.597(1000 μg / m L); the enzymatic hydrolysis had the highest percentage of components with relative molecular mass 〈1 000,to 74.11%.