中文摘要：

以7-二乙胺基香豆素-3-羧酸、甘氨酸、组氨酸为原料，采用标准多肽合成法成功合成了水溶性的荧光探针分子香豆素-甘氨酸-甘氨酸-组氨酸（Coumarin-Gly-Gly-His，C-GGH），利用GGH三肽对Cu2＋的络合作用形成C-GGH-Cu2＋络合物，使探针分子C-GGH的荧光猝灭；然后，通过向体系中加入氰化物后，CN-对Cu2＋的强络合作用形成稳定的络合物[Cu（CN）x]而使探针分子荧光逐渐恢复，以此实现对氰化物的定量检测。该法检测限为0．015μmol／L，远低于国家标准（GB85372008）中要求最严的矿泉水中氰化物的限量值0．01mg／L（相当于摩尔浓度0．385μmol／L），该探针分子对CN-的检测不受SCN-、F-、Cl-、Br-、I-、PO3- 4、N3-、NO3-、AcO-、SO2- 4、CO 2- 3等共存阴离子的影响。经对实际食物样品中氰化物进行检测，证明该方法的准确度与国标（GB／T5009．48-2003、GB／T5009．36-2003、GB／T8538-2008）方法一致，且更快捷、简单。

英文摘要：

Cyanide is considered to be one of the most toxic species in food while the existing detection methods involve complex operationand will produce toxicity to inspectors. In this study, a water-soluble fluorescent probe（C-GGH） was synthesized by HOBT/DIC protocol in solution using 7-diethylaminoeoumarin-3-carboxylie acid, Glyeine and Histidine. Due to the presence of the recognition unit GGH, the probe C-GOH can conjugated with Cu2＋ to formed non-fluorescent C- GGH Cu2＋ complex. Due to the strong binding affinity of CN- to Cu24, CN can extract Cu2＋from C--GGH-Cu2＋complex, leading to the release of C-GGH and the recovery of fluorescent emission of the system. The probe allowed detection of cyanide in aqueous solution with a LOD （limit of detection） of 0.017 μmol/L which is much lower than the maximum contaminant level （0.385μmol/L） for cyanide in mineral water set by national standard （GB 8537 2008）. The probe also displayed excellent specificity for CN-towards other anions, including F -, Cl- , Br- , I- , SCN- , PO3- 4 , N3- , NO3- , AcO- , SO2- 4 , and CO2-3. The method developed in this study received the same results as national standard method （GB/T 5009.48 2003, GB/T 5009.36-2003, GB/T 8538-2008）, but more simple and quick in real sample detection.