针对对氯硝基苯和硝酸盐复合污染问题,以乙醇作为共基质,通过间歇式实验,在基质充足和不足2种条件下考察了NO3-对对氯硝基苯还原过程影响。实验结果表明,在基质充足条件下,对氯硝基苯和NO3-还原过程不互相产生抑制作用。当初始COD浓度为100 mg/L,由于基质不足,NO3-对对氯硝基苯还原过程产生竞争性抑制作用,且抑制作用随着NO3-浓度升高而增强,当NO3-浓度为150 mg/L和300 mg/L时,与空白样相比对氯硝基苯还原速率分别下降20%和54%,且溶液中出现NO2-积累,浓度分别为33.68 mg/L和44.92 mg/L。当采用生物法修复氯硝基苯化合物和硝酸盐复合污染水体时,应考虑基质的供给和硝酸盐的影响。
The effect of NO3 on para-chloronitrobenzene (p-CNB) reduction was investigated under the conditions of sufficient substrate and insufficient substrate with batch experiments due to the combined pollution of p-CNB and NO3-. Ethanol was used as co-substrate. Both p-CNB reduction and NO3- reduction proceeded successfully with sufficient substrate. However, p-CNB reduction was competitively inhibited by NO3- when sub- strate was insufficient (COD = 100 mg/L). The inhibiting effect enhanced as the NO3- concentrations increased. p-CNB reduction rate decreased by 20% and 54% when compared to the control, as initial NO3- concentrations were 150 mg/L and 300 mg/L, respectively. Nitrite accumulation was also observed with a concentration of 33.68 mg/L and 44.92 mg/L in the above tests. It indicated that the effects of co-substrate and nitrate should be taken into account when anaerobic biological treatment is applied for the remediation of chloronitrobenzenes con- taminated waters.