中文摘要：

为了研究桉树不同无性系在铝处理下的叶片蛋白表达的差异,并从分子生物学角度预测与阐释桉树耐铝性机制,以巨尾桉（Eucalyptus grandis×E.urophylla）9号、巨尾桉（E.grandis×E.urophylla）12号、韦赤桉（E.wetarensis×E.camaldulensis）3号和尾叶桉（E.urophylla）4号苗木为材料,并对其进行铝处理。结果表明：从4种无性系叶所得蛋白有明显的上调或下调表达。通过丰度计算,共得蛋白点（P〈0.05）98个,巨尾桉9号为14个、巨尾桉12号为38个、尾叶桉4号为33个、韦赤桉3号为13个。经质谱分析,有11个蛋白成功鉴定,包括1个Ru Bis CO、3个Ru Bis CO LSU、1个放氧增强蛋白、1个叶绿素a/b结合蛋白、3个2-半胱氨酸过氧化物酶、1个质体谷氨酰胺合成酶、1个3-磷酸甘油醛脱氢酶,其主要功能与植物自身N和能量代谢以及抗氧化和光合作用等有关。4种不同的桉树无性系应对铝胁迫时有不同的应答机制,差异蛋白质呈现不同的表达量,并且从该角度分析耐铝性最强的是巨尾桉9号,而较弱的是尾叶桉4号。

英文摘要：

In order to explore the differences of protein expression in the leaves of different clones of Eucalyptus under aluminum treatment and predict and explain the mechanism of the resistance to aluminum from the perspective of molecular biology,the study analyzed the expression of leaf differential proteins with E. grandis × E. urophylla clone No. 9 and No. 12,E. wetarensis × E. camaldulensis clone No.3 as well as E. urophylla No. 4 as the trial materials which were treated by aluminum. The results showed that the protein expression of 4 species of Eucalyptus clones was significantly up-regulated or down-regulated. Via the abundance calculations,there were 98 proteins（ P〈0. 05）,among them,E.grandis × E. urophylla clone No. 9 obtained 14 differential protein spots,E. grandis × E. urophylla clone No. 12 obtained 38 differential protein spots,E. urophylla clone No. 4 obtained 33 differential protein spots and E. wetarensis × E. camaldulensis clone No. 3 obtained 13 differential protein spots.Eleven proteins were successfully identified by mass spectrometry analysis,including 1 Ru Bis CO,3Ru Bis CO LSU,1 oxygen-evolving enhancer protein,1 chlorophyll a/b binding protein,3 2-cystenine peroxidase,1 plastid glutamine synthetase,1 glyceraldehydes 3-phosphoric acid dehydrogenase,which were mainly related to the role of antioxidant,photosynthesis,nitrogen metabolism and energy metabolism. The 4 Eucalyptus clones exhibited different response mechanism under aluminum treatment,and E.grandis × E. urophylla clone No. 9 showed the strongest aluminum resistance,while E. urophylla clone No. 4 showed the weakest resistance.