中文摘要：

客观的猪的肝摘录被显示了在严重肝炎的临床的处理有效。现在的学习的目的是在 vitro 学习它的 antifibrotic 以及有免疫力的规章的效果。方法 Hepatocytes，肝的星形的房间(HSC ) ， hepatoma 房间线(HepG2 ) 和人的外部血 mononuclear 房间(PMNC ) 被增长试金，放射性免疫测定，基因 transfection，记者基因分析和流动 cytometry 分别地关于增长，细胞外的矩阵生产和 apoptotic 活动学习。结果强壮的 stimulatory 增长效果在 hepatocytes 被观察，并且禁止的效果在 HSC 被发现。玻尿的酸(哈) 开车由的生产和记者基因活动各种各样在 HSC-T6 的 1 个(I) procollagen 基因倡导者显著地与摘录在处理以后被减少。Fluo-Anexin V 有约束力的 apoptotic HepG2 房间面对 60 g/ml 摘录是更突出的。更多的 CD4+/CD69+ 积极 T 淋巴细胞面对摘录存在。结论猪的肝摘录为经由在 vitro 的 hepatocyte 新生， HSC 和 hepatoma 房间抑制的 antifibrogenesis 是有效的。活跃 T 淋巴细胞的举起对有免疫力的监视有用。摘录的好印射是必要的以便得到在所有描述的功能必要的明确的分子。

英文摘要：

Objective： Porcine liver extract has been shown to be effective in the clinical treatment of severe hepatitis. The aim of the present study was to study its antifibrotic as well as immune regulatory effect in vitro. Methods： Hepat＇ocytes, hepatic stellate cells （HSCs）, hepatoma cell line （HepG2） and human peripheral blood mononuclear cells （PMNCs） were studied with respect to proliferation, extracellular matrix production and apoptotic activities by proliferation assay, radioimmunoassay, gene transfection, reporter gene analysis and flow cytometry, respectively. Results： A strong stimulatory proliferation effect was observed in hepatocytes, and an inhibitory effect was found in HSCs. Hyaluronic acid （HA） production and reporter gene activities driven by various etl（I） procollagen gene promoters in HSC-T6 were significantly decreased after treatment with the extract. Fluo-Anexin V binding apoptotic HepG2 cells were more prominent in the presence of 60ug/ml extract. More CD4^＋/CD69^＋ positive T lymphocytes existed in the presence of the extract. Conclusion： Porcine liver extract is effective for antifibrogenesis via hepatocyte regeneration, HSC and hepatoma cell inhibition in vitro. The elevation of active T lymphocytes is helpful for immune surveillance. Fine mapping of the extract is necessary in order to get definite molecules which are essential in all described functions.