中文摘要：

目的：通过构建SLOOP慢病毒表达载体，研究其对结肠癌Caco-2细胞的影响。方法：以DLD-1细胞cDNA为模版，PCR扩增SLOOP基因序列，然后将该序列克隆插入慢病毒载体中，构建SLOOP慢病毒表达载体，包装产生慢病毒颗粒。将慢病毒颗粒感染Caco-2细胞，荧光显微镜观察细胞绿色荧光蛋白表达；应用逆转录聚合酶链反应（RT—PCR）和蛋白印迹（Western blot）技术检测SLOOP的表达情况；MTT法检测细胞生长变化；细胞平板克隆培养检测其细胞克隆形成能力。结果：构建的慢病毒表达载体Lenti～SLOOP经酶切和测序鉴定结果正确；携带SLOOP基因的慢病毒颗粒感染Caco．2后，细胞中SLOOP基因和蛋白过表达，促进细胞克隆形成能力。结论：本研究成功构建SLOOP慢病毒表达载体，为深入研究SLOOP基因的相关功能提供了一定的实验基础。

英文摘要：

Objective： To construct a lentiviral vector containing SLOOP gene and study its effect on colon cancer Caco-2 cells. Methods： SLOOP was amplified from cDNA of DLD-1, and inserted into lentiviral expression vector to generate Lenti-S100P vector. The inserted SLOOP gene was verified by double enzyme digestion and DNA sequencing. Subsequently, lentiviruses were produced and transduced into Caco-2 cells. EGFP expression was examined under fluorescence microscopy, the expression of SLOOP was detected with semi-RT-PCR and Western blot. Cell growth and colony forming ability were detected with MTT and plate cloning technique. Results： The lentiviral S 100P expression vector was successful constructed. Overexpression of S 100P was verified in Caco-2 cells with Lenti-S 100P. S 100P promoted Caco-2 cells colony forming ability, while no signifi- cant effect on cell growth. Conclusion： Lenti-SlOOP vector is successfully constructed, and it will provide a basis for further study of SLOOP gene function.