中文摘要：

目的：基于Notch4和Delta4探讨当归补血汤（Danggui Buxue Decoction,DBD）协同肌源性干细胞（Musclederived stem cells,MDSCs）向造血方向调控的作用机制。方法：将雌性昆明小鼠随机分成6组,分别为照射模型组、骨髓移植组、MDSCs移植组、等效剂量DBD预处理＋MDSCs组（给药1组）、3倍DBD预处理＋MDSCs组（给药2组）和5倍DBD预处理＋MDSCs移植组（给药3组）。照射前各组分别给予生理盐水和不同剂量DBD灌胃1周,经8Gy~（137）Cs-γ射线照射后,尾静脉分别移植骨髓细胞和MDSCs。用real-time PCR检测3周末时小鼠骨髓中CD34、Notch4和Delta4 mRNA表达量。结果：与照射模型组比,干预组中CD34 mRNA上调（P〈0.05）,且以给药3组上调幅度最大;Notch4 mRNA下调（P〈0.05）,且给药1组下调幅度最大;除骨髓移植组外,其余各组Delta4 mRNA均上调（P〈0.05）,且给药3组上调幅度最大。结论：当归补血汤协同肌源性干细胞移植能促进受照射小鼠造血重建,且5倍当归补血汤协同MDSCs移植效果最佳。Notch4和Delta4在骨髓造血干细胞移植和MDSCs移植中的作用不同,其促进骨髓造血干细胞、MDSCs向造血细胞分化的具体调控机制尚待深入探索。

英文摘要：

Objective： To explore the molecular mechanism of Notch4 and Delta4 in the process of muscle-derived stem cells（ MDSCs） differentiation into hematopoietic cells after MDSCs had been transplanted to mice which have been pretreated with Danggui Buxue Decoction（ DBD）. Methods： Female mice were randomly divided into 6 groups,including irradiation model group,bone marrow cell transplantation group,muscle-derived stem cell transplantation group,equivalent dose DBD ＋ MDSCs transplantation（ dosing group 1）,3 times dose DBD ＋ MDSCs transplantation（ dosing group 2） and five times dose DBD ＋ MDSCs transplantation（ dosing group 3）. All the mice were pretreated by normal saline or different doses of DBD through gavage for seven days. And then the bone marrow cells and muscle-derived stem cells were transplanted into caudal veins after radiated by137Cs-gamma ray,and detect the expressions of CD34 mRNA,Notch4 mRNA and Delta4 mRNA by real-time quantitative PCR.Results： Real-time PCR results showed that as compared with the irradiation model group,the expression levels of CD34 were significantly increased in intervention groups（ P〈0. 05）. The expression level of CD34 in the dose group 3 was the highest. The expression levels of Notch4 were significantly decreased in intervention groups（ P〈0. 05）. The expression level of Notch4 in the dose group 1 was the lowest. Except the bone marrow cell transplantation group,the expression levels of Delta4 were significantly increased,and those of the dose group 3 were the highest. Conclusion： DBD pretreatment combination with MDSCs transplantation can re-establish the hematopoietic function of receptor mice irradiated. Especially the five times dose DBD ＋ MDSCs transplantation have the best effect on hematopoietic reconstruction of recipient mice irradiated. In addition,Notch 4 and Delta 4 play different roles in the process of bone marrow cells transplantation and DBD pretreatment combination with MDSCs transplantation. The mechanism of both bon