中文摘要：

目的：观察活性维生素D3腹腔注射对2型糖尿病大鼠海马神经元的影响.方法：SD大鼠30只,随机分为空白对照组（Control）,高脂组（HF）,高脂＋VD组（HF＋VD）,糖尿病组（DM）,糖尿病＋VD组（DM＋VD）.测定大鼠空腹血糖、胰岛素、维生素D等生化指标,应用透射电镜观察海马神经元结构变化.结果：与对照组相比,HF组、HF＋VD组大鼠体重增加,（分别为343.28±10.41 g;356.81± 36.20 g和360.18±25.56g）,DM组体重降低（265.6±16.11 g）（P＜0.05）;DM组SDF-1为398.33±15.01 ng/L,低于Control组（457.53±26.56 ng/L）,骨化三醇干预后SDF-1显著升高（448.54±36.83 ng/L）（P＜0.05）;DM组（25-OH-D）水平为165.25±30.75 μg/L,低于Control组213.65±30.79 μg/L,补充骨化三醇后显著提高至210.31±50.69 μg/L（P＜0.05）;DM组海马神经元CXCR4表达降低,线粒体可见明显肿胀,DM＋VD组线粒体形态明显改善,海马神经元CXCR4表达水平明显升高.结论：常规量补充维生素D可提高糖尿病大鼠体内维生素D水平,对海马神经元有明显保护作用.

英文摘要：

Objective：To explore the effect of vitamin D3 on the hippocarpal neurons of type 2 diabetic rats.Methods：30 female SD rats were randomly divided into control group,high fat group （HF）,high fat ＋ vitamin D （HF ＋ VD）,diabetes mellitus group （DM）,diabetes mellitus ＋ vitamin D （DM ＋ VD）.The blood sugar,insulin and vitamin D level were detected at the beginning and end of the experimental period.The hippocampal neurons were observed by transmission electron microscope.Results：Compared with control group （343.28± 10.41 g）,body weight of HF （356.81± 36.20 g）,HF ＋ VD rats （360.18± 25.56 g） increased and that of DM rats （265.6± 16.11 g）decreased（P〉0.05）.SDF-1 decreased in diabetic rats（398.33± 15.01 ng/L）.calcitriol treatment reversed the changes significantly （448.54± 36.83 ng/L）（P〈0.05）.25-OH-D decreased in DM rats （165.25± 30.75 μg/L）and calcitriol treatment increased 25-OH-D in DM rats 210.31 ± 50.69 μg/L （P〈0.05）.The expression of CXCR4 decreased in hippocampal neurons of DM rats,and swelled mitochondria was observed in the neurons.calcitriol treatment improved the injury.Conclusion：calcitriol treatment increased vitamin D level,and has protective effect on hippocampal neurons in type 2 diabetes mellitus rats.