中文摘要：

为了对DNA损伤诱导蛋白1（Ddi1-like）基因功能进行研究,本研究参考GeneDB中猪带绦虫基因组注释信息,通过设计5′和3′端特异性引物,利用cDNA末端快速扩增法（RACE）获得全长cDNA,并在毕赤酵母GS115中进行诱导表达;将纯化后的Ddi1-like重组蛋白免疫新西兰大白兔,制备抗血清。结果,成功利用RACE获得Ddi1-like基因的全长cDNA序列;在真核表达系统中,绦虫上的Ddi1-like重组蛋白获得表达;SDS-PAGE和Western-blot分析表明,获得重组蛋白即为Ddi1-like蛋白;免疫后获得抗血清,其效价达到1∶12 800。上述结果为后续研究Ddi1-like蛋白的酶切活性研究提供了基础。

英文摘要：

To functionally study the DNA damage-inducible-like （Ddil-like） protein from Teania solium,according to gene annotations of K solum genome in GeneDB,the cDNA of Ddil-like gene was ampli- fied by RACE （rapid-amplification of cDNA ends） technique using the specific 5＇and 3＇primers,and was then cloned into vecter pPIC9K. Then the recombinant protein was induced in E pastoris. New Zealand white rabbits were immuned by the purified recombinant Ddil-like protein to produce the anti-serum. In result,the full ORF of Ddil-like gene was successfully obtained. The recombinant Ddil-like protein was produced in~pastoris expression system. The recombinant protein expression was confirmed by SDS-PAGE and Western-blot analysis. The antibody titer of the anti-serum could reach to 1：12 800. The above-men- tioned results lay a foundation to further studies on the proteolytic activity of the Ddil-like protein.