中文摘要：

为进一步阐明大蓟的化学成分,运用硅胶、大孔吸附树脂（Diaion-101）、Sephadex LH-20等柱色谱和薄层色谱技术对大蓟进行提取分离,根据波谱数据和参考文献等手段从中鉴定了9个木脂素类化合物,分别为：爵床脂素B（1）,6＇-羟基爵床脂素A（2）,6＇-羟基爵床脂素B（3）,山荷叶素（4）,爵床脂素A（5）,6-羟基-4-（4-甲氧基-3-甲氧基苯基）-3-羟甲基-5-甲氧基-3,4-二氢（3R,4S）-2-醛基萘（6）,6-羟基-4-（4-甲氧基-3-甲氧基苯基）-3-羟甲基-7-甲氧基-3,4-二氢（3R,4S）-2-醛基萘（7）,4＇-去甲基鬼臼毒素（8）,鬼臼毒素（9）.化合物（1～9）均为首次从大蓟中分离得到.同时,本研究用D-氨基半乳糖对HL-7702细胞诱导产生毒性,采用MTT法对化合物（1～9）进行肝保护活性筛选,结果表明化合物2,3,4,7和8（10μm）具有一定的肝保护活性.

英文摘要：

To investigate the chemical constituents of Cirsium japonicum,nine lignans were obtained by column chromatography on silica gel,macroporous resin（ Diaion-101）,Sephadex LH-20,and TLC for separation and purification. Their structures were identified as justicidin B（ 1）,6＇-hydroxy justicidin A（ 2）,6＇-hydroxy justicidin B（ 3）,diphyllin（ 4）,justicidin A（ 5）,6-hydroxy-4-（ 4-methoxyl-3- methoxyphenyl）-3-hydroxymethyl-5-methoxyl-3,4-dihydro（ 3R,4S）-2-aldehydenaphthalene（ 6）,6-hydroxy-4-（ 4-methoxyl-3-methoxyphenyl）-3-hydroxymethyl-7- methoxyl-3,4-dihydro（ 3R,4S）-2-aldehydenaphthalene（ 7）,4＇-demethyl podophyllotoxin（ 8）,podophyllotoxin（ 9） by spectroscopic data and references. Compounds（ 1 ~ 9） were isolated from this plant for the first time. Meanwhile,Compounds（ 1 ~ 9） were evaluated for their hepatoprotective activities against D-galactosamine-induced toxicity in HL-7702 cells using a MTT colorimetric method.