中文摘要：

目的 ：观察microRNA-29c（miR-29c）在大鼠单侧输尿管梗阻（UUO）模型肾组织中的表达变化,探讨其是否参与肾间质纤维化及其可能机制。方法：高通量测序技术和实时荧光定量PCR检测miR-29c在UUO大鼠术后1 d和3 d肾脏组织中的表达情况。使用生物信息学预测miR-29c靶mRNA,对其靶mRNA进行Gene Ontology和pathway分析。结果：UUO大鼠术后1 d和术后3 d手术侧肾组织miR-29c表达下调,Col I mRNA和FN mRNA的表达升高。预测靶mRNA经分析后获得miR-29c有统计学意义的10个靶mRNA,其中6个编码细胞外基质蛋白。结论：miR-29c在大鼠UUO模型早期的肾组织中表达下调,可能通过促进细胞外基质堆积参与肾间质纤维化的发生发展。

英文摘要：

Objective： To observe the change of expression pattern of miR-29c in kidney of unilateral ureteral obstruction（UUO） rats and discuss whether miR-29c take part in renal interstitial fibrosis（RIF） and its probable mechanism.Methods： High-throughput sequencing and real-time quantitative PCR analysed the expression of miR-29c in the kidney of UUO rats,which were killed 1 and 3 days after operation.The miR-29c targets were predicted by bioinformatics and then analysed with Gene Ontology analysis and pathway analysis.Results： The expression of miR-29c decreased,while the expression of Col I mRNA and FN mRNA increased in the surgical kidney of UUO rats.Ten miR-29c targets with statistical significance were obtained after analysis of thom,6 targets coded were extracellular matrix proteins.Conclusion： The expression of miR-29c decreased in the kidney of early UUO rat model,miR-29c may participate in RIF induced by UUO through promoting extracellular matrix accumulation.