中文摘要：

以抗赭曲霉毒素A的单克隆抗体亲和筛选随机7肽库,ELISA方法鉴定阳性克隆,DNA测序推导出赭曲霉毒素A的模拟表位肽,共获得了11个模拟表位肽,共有序列为IRPMV。将化学合成的模拟表位肽与载体BSA偶联后,以之建立无毒素的竞争酶联免疫检测方法,并与常规酶联免疫吸附检测方法及高效液相色谱比较。结果表明：以赭曲霉毒素A模拟表位建立的竞争ELISA方法的线性范围和检测下限都与常规竞争ELISA方法结果相近,样品测试结果与常规竞争ELISA方法及高效液相色谱一致,显示良好的应用前景。

英文摘要：

An anti-OTA monoclonal antibody was used to screen mimotope peptides from a phage random 7peptide library.The positive clones were identified by ELISA.Mimotopes were deduced by DNA sequencing.11mimotope peptides were acquired.The common amino sequence was IRPMV.A competitive ELISA was set up with a mimotope peptide conjugated to BSA carrier,and the new method was compared with conventional ELISA method and HPLC method. In the new established method,the linear range of the inhibition curves and the detecting limitation were similar to those of conventional ELISA method.The sample test results were identical to the conventional ELISA and HPLC,which implies a promising applica-tion.