以羽衣甘蓝圆叶自交系‘0835’和裂叶自交系‘0819’为亲本,调查P1、P2、F1、F2群体莲座期4个裂叶相关性状表型数据,运用‘四世代主基因+多基因’遗传模型,对叶长、叶宽、叶形指数、叶缘缺刻数4个叶形相关性状进行遗传分析,探讨羽衣甘蓝裂叶相关性状的遗传规律,为羽衣甘蓝裂叶性状遗传、QTL定位及新品种选育奠定基础。结果表明:(1)4个性状均存在一定的杂种优势,其中叶缘缺刻数中亲优势达显著水平,4个性状均存在负向超亲优势。(2)叶长和叶宽均符合E-4模型,即由2对等加性主基因+加性-显性多基因共同控制;叶长主基因遗传率为83.80%,多基因遗传率为1.05%;叶宽主基因遗传率为22.28%,多基因遗传率为61.92%。(3)叶形指数和叶缘缺刻数均符合E-1模型,即由2对加性-显性-上位性主基因+加性-显性多基因控制;叶形指数主基因遗传率为93.73%,多基因遗传率为2.59%;叶缘缺刻数主基因决定了表型变异的91.18%。
We constructed an F2 genetic segregation population crossed by parents between a flat-leaved inbred line‘0835’and a feathered-leaved inbred line‘0819’in ornamental kale(Brassica oleracea var.acephala).Four feathered-leaved related traits,i.e.leaf length,leaf width,leaf index and number of serrated leaf margin,were investigated at rosette stage.The genetic segregation analysis was conducted by using four generations(P1,P2,F1 and F2)mixed major gene plus polygene inheritance model.The results showed that:(1)there are prominent helerosis in the trait of number of serrated leaf margin.Meanwhile,these four characters showed negative transgressive heterosis.(2)Genetic analysis of quantitative traits indicated that leaf length and leaf width were both compatible with E-4model characterized by two equal additive major gene plus additive-dominant polygene.The heritabilities of major gene and polygene were 83.80% and 1.05%,respectively,in leaf length.The heritabilities of major gene and polygene were 22.28% and 61.92%,respectively,in leaf width.(3)Leaf index and number of serrated leaf margin both meet E-1model showing two additive-dominant-epistatic major gene plus additive-dominant polygene.The heritabilities of major gene and polygene were 93.73% and 2.59%,respectively,in leaf index.The major gene heritability was91.18%in number of serrated leaf margin trait.