大豆细菌性斑点病是江淮地区大豆生产中常见病害,但大豆种质资源抗性水平及抗源鉴定工作较少。本研究采用对大豆叶片正反面高压喷雾的接种方法鉴定了江淮地区309份育成品种(系)及亲本材料对大豆细菌性斑点病生理小种S1的抗感反应。结果表明:供试材料抗性差异明显,分别鉴定出高抗和中抗材料61和68份,占总数的19.74%和22.1%,表现为感病和高感的材料共有180份,占总数的58.25%。适合淮北和淮南地区种植的140和169份品种(系)中,抗病材料(高抗+中抗)分别有68和61份,感病材料(感病+高感)分别有72和108份,江淮淮北地区抗病品种(系)的比例高于淮南地区。供试材料抗性反应等级与成熟期等性状存在相关性。同时还发掘出徐豆18、南农99.6等高抗品种,及具有高蛋白、高油特性的优质抗性种质材料。
Bacterial leaf blight is one of the common diseases in soybean production in the Yangtze and Huaihe River Valleys. However, the resistance performance of soybean germplasm in this region remains unclear. In the present study, the resistance levels of 309 selected soybean varieties or breeding lines to Pseudomonas syringae pv-glycinea S1 strain were evaluated by inoculating both sides of leaves through spray method in field condition.The results showed that there were significant resistance differences in the sample 61 (19.74%) and 68 (22.1%) varieties or breeding lines were identified with high and medium resistance levels respectively, a total of 180(58.25%) varieties(lines) of the sample were found with susceptible or highly susceptible level. In the sample, 68 and 72 varieties(lines) were identified as resistant and susceptible in northern Huaihe River region respectively, and 72 and 108 varieties(lines) were identified as resistant and susceptible types in southern Huaihe River region respectively. The ratio of resistance varieties(lines) to Pseudomonas syringae pv. glycinea from Northern Huaihe River regions was higher than that form the southern region, and significant correlation between the disease resistance grade and maturity was found. Some high-resistant varieties(lines) including Xudou 18 and Nannnong 99.6 and some high-resistant lines with high seed protein content or oil content were identified and could be used in the resistance breeding and prevention of Pseudomonas syringaepv.glycinea in soybean