位置:成果数据库 > 期刊 > 期刊详情页
蓖麻杂交种的SSR鉴定及遗传变异分析
  • ISSN号:1000-4025
  • 期刊名称:西北植物学报
  • 时间:2012.8.8
  • 页码:1539-1546
  • 分类:Q789[生物学—分子生物学] Q346.5[生物学—遗传学]
  • 作者机构:[1]中国农业科学院油料作物研究所,农业部油料作物生物学与遗传育种重点实验室,武汉430062
  • 相关基金:国家自然科学基金(31000726);农业部公益性行业(农业)科研专项(201003057);中国农科院油料所所长基金(1610172011009)
  • 相关项目:蓖麻矮秆性状的遗传分析及QTL初步定位
中文摘要:

采用SSR标记对蓖麻CSR24×CSRl81杂交所得的F1种子进行分析,为蓖麻早期杂种鉴定和遗传变异分析奠定技术与理论基础。结果表明:(1)各位点鉴定所得结果基本一致,除RCM207和RCl29位点鉴定的杂种率未超过10%外,其它位点的杂种率都十分接近,在13.46%-17.27%之间。(2)少数个体在相关位点发生了变异,在引物RC242的扩增图谱中有4个单株出现了双亲特异条带的缺失,产生了双亲都没有的新条带;在引物Rc023、Rc026、Rc029、RCl29、RCM613和RCM999的扩增结果中出现了父本特异条带的缺失,同时产生了一条新条带。(3)多样性及UPGMA聚类分析表明杂交后代遗传变异显著,子代个体与亲本间的遗传相似系数在0.45-1.0之间,个体间差异明显。

英文摘要:

In order to identify the hybrid purity of castor bean in early seedling stage or before sowing,cas- tor bean hybrid verification and genetic diversity of F1 seeds derived from CSR24 × CSR181 hybridization were performed by SSR markers. It was showed that the results by different loci were basically consistent, and the hybrid rates estimated by most loci were from 13. 46% to 17. 27%, only which evaluated by RCM207 and RC129 were less than 10%. The variation was occurred on some loci of several individuals,4 progenies had non-parent markers and generated two new bands in primer RC242; the paternal special bands were absent and a new band was appeared in some progenies in primer Rco23 ,Rco26 ,Rco29 ,RC129, RCM613 and RCM999. The genetic diversity and cluster analysis of SSR data indicated that the distinct differentiation existed in FI progenies,the genetic variation was obvious,and genetic similarity coefficient between parents and progenies was 0.45-1.0. It was provided a method for early verification of true hybrids and offered the theoretical reference for genetic analysis of offsprings.

同期刊论文项目
同项目期刊论文
期刊信息
  • 《西北植物学报》
  • 北大核心期刊(2011版)
  • 主管单位:中华人民共和国教育部
  • 主办单位:西北农林科技大学 陕西省植物学会
  • 主编:赵忠
  • 地址:陕西杨陵邰城路3号西北农林科技大学
  • 邮编:712100
  • 邮箱:xbzwxb@vip.163.com
  • 电话:029-87082936
  • 国际标准刊号:ISSN:1000-4025
  • 国内统一刊号:ISSN:61-1091/Q
  • 邮发代号:52-73
  • 获奖情况:
  • 全国优秀期刊,中国自然科学核心期刊,中国期刊方阵“双效”期刊
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,美国生物科学数据库,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:46104