中文摘要：

目的 研究调节性T细胞（Tr）和Th17细胞在特异性NF-κB抑制诱导同种异体小鼠心脏移植耐受的早期作用机制.方法 以BALB/c小鼠为供体,C57BL/6小鼠（对照组）和IκBα△N-Tg小鼠（实验组）分别作为受体建立同种异体腹部异位心脏移植模型;流式细胞术分别检测两组受鼠脾脏内Tr在移植前以及移植术后7 d、30 d和100 d的变化规律,以及Th17细胞在移植术后5 d时的变化;Western blot检测两组心脏移植物内IL-17在移植后3 d和5 d的表达.结果 实验组心脏移植物存活时间均大于100 d,HE染色未见心脏移植物内有明显淋巴细胞浸润;实验组Tr比例在移植后7 d和30 d时明显升高（21.23±3.95,23.17±4.11 vs 11.64±1.96,P〈O.05）,但是100 d时Tr细胞比例又下降到了移植前水平（10.79±2.48 vs 11.64±1.96,P〉0.05）,而对照组Tr在移植前后则无明显变化;与对照组相比,实验组Th17细胞及移植物内IL-17表达在移植术后5 d时均明显下降.结论 特异性受体T细胞NF-κB功能缺陷可以打破受体内Tr/Th17细胞平衡,在移植术后早期促进T细胞向Tr分化而抑制向Th17细胞分化,从而阻止急性排斥反应发生,诱导耐受.

英文摘要：

Objective To investigate whether allocardiac graft acceptance in the specific NF-κB impaired mice is due to regulatory T cell（Tr） and Th17 cells.Methods Mice abdominal heterotopic cardiac transplantation was performed and then divided in to control group（BALB/c→C57BL/6） and experimental group（BALB/c→IκBα/△N-Tg）.Pretransplant and at day 7,30,100 posttransplant,spleens were harvested from the IκBα△ N-Tg mice,and then the Tr were detected by the fluorescence activated cell sorter.At day 5 posttransplant,the CD4 ＋ Th17 cells from the spleens of the two groups were examined by the FACS.Additionally,at day 3 and 5 posttransplant,IL-17 expressed in the cardiac allograft was detected by Western blot.Results In the IκBα/ N-Tg mice group,the cardiac allografts were survived more than day 100,and without obviously lymphocytes infiltration.At the day 7 and 30 posttransplant,the Tr was obviously increased（21.23 ± 3.95,23.17 ± 4.11 vs 11.64 ± 1.96,P 〈 0.05）; however,the Tr decreased at the day 100 posttransplant,and had no difference with before transplant（10.79 ±2.48 vs 11.64 ± 1.96,P 〉0.05）.Compared with the control group,at day 5 posttransplant,CD4＋ Th17 cells in the IκBα/N-Tg mice and IL-17 expression of the cardiac allograft were both decreased.Conclusion In the early stage after transplantation,specific T cell NF-κB impaired could abrogate the balance of the Tr and Th17 cells,and induce the T cells differentiated into Tr and inhibit the Th17 cells differentiation,and then induce tolerance.