中文摘要：

目的：比较添加葡萄糖或蔗糖的冷冻保护剂对人类精子冷冻复苏后活动力的影响。方法：将50例志愿者的精液标本分别采用添加葡萄糖或蔗糖的保护剂冷冻，比较精子复苏后的活动力。结果：冷冻前，前向活动精子百分率为（58.4±5.7）％，活动率为（63.4±6.1）％，采用添加葡萄糖的冷冻保护剂冷冻复苏后的前向活动精子百分率为（43.8±7.6）％，活动率为（48.4±7.6）％；采用添加蔗糖的冷冻保护剂冷冻复苏后的前向活动精子百分率为（42.6±8.9）％，活动率为（48.0±8.5）％。冷冻前后精子的前向活动百分率和总活动率差异有统计学意义（P〈0.01），采用2种不同的冷冻保护剂冷冻复苏后的精子前向活动百分率和总活动率没有统计学差异（P〉0.05）。结论：冷冻对精子活动力损伤明显，添加蔗糖作为精子冷冻的保护剂是可行的。

英文摘要：

Objective： To compare the effects of the cryoprotectant containing glucose and that containing sucrose on the motility of post-thaw human sperm, Methods： The cryoprotectant containing glucose and that containing sucrose were applied to 50 semen sam-ples and the motility of the post-thaw human sperm was compared before and after cryopreservation and between the study groups. Resuits ： The forward motility and total motility of the sperm were （ 58.4±5.7 ） % and （ 63.4±6.1 ） % before cryopreservation, （ 43.8±7.6 ） % and （ 48.4±7.6 ） % after thawing with the cryoprotectant containing glucose, and（ 42.6±8. 9 ） % and （ 48.0±8. 5 ） % after thawing with the cryoprotectant containing sucrose, Decreased sperm motility was observed after cryopreservation, with statistic significance （ P 〈0.01 ）. There was no significant difference in the forward and total motility of the post-thaw sperm between the two cryo- Drotectants. Conclusion： Cryopreservation inflicts obvious damage on sperm. Sucrose is a feasible sperm cryoprotectant.