中文摘要：

目的：利用荧光探针JC-1观察氧化应激对精子线粒体膜电位的变化。方法：用FeSO4/H2O2体系诱导精子氧化损伤模型，利用新型荧光探针JC-1标记精子线粒体，在FACS Calibur流式细胞仪上检测JC-1荧光强度的变化。结果：正常精子线粒体维持较高的膜电位，JC-1在线粒体内形成聚合物，红色荧光占主导地位。VeSO4／H2O2体系导致线粒体膜电位下降，JC-1聚合物分解成单体，红色荧光强度减弱。结论：氧化应激导致精子线粒体膜电位下降；JC-1结合流式细胞仪是一种理想的检测线粒体膜电位的手段。

英文摘要：

Objective： To explore the changes of mitochondrial membrane potential（MMP） in sperms in oxidative stess model with fluorescent JC-1. Methods： FeSO4/H2O2 system was used to induce sperm model of oxidative injury, and JC-1 in combination with flow cyometry was used to detect the changes of sperm MMP in the exposure of reactive oxygen species（ROS）. Results： Normal sperms had a high MMP, and JC-1 aggregates were formed in the inner membranes of mitochondria and emitted orange-red fluorescence. ROS caused the decrease of MMP, and JC-1 aggregates were dissociated to monomer which emitted green fluorenscence. Conclusions： Oxidative stress can cause the decrease of MMP; JC-1 in combination with flow eytometry is an ideal method to detect the change of mitochondrial membrane potential.