中文摘要：

采用HPLC-UV法研究不同比例黄药子-当归配伍组合中黄独乙素及儿茶素的质量分数.采用95％乙醇对黄药子-当归配伍组合进行提取,经乙酸乙酯萃取后,测定黄药子及各配伍组合中黄独乙素及儿茶素质量分数.结果表明黄独乙素和儿茶素分别在10～200μg/mL及200～1 200μg/mL范围内呈良好线性关系,平均回收率分别为89.4％及92.8％.黄药子单煎液中黄独乙素质量分数2.90 mg/g,儿茶素质量分数为0.78 mg/g,黄药子-当归以1∶1,1∶2,2∶1配伍时,各组中黄独乙素质量分数分别为1.01,2.19,2.04 mg/g,表明黄药子与当归以1∶1比例配伍后,致肝毒性成分黄独乙素质量分数最低.各组中儿茶素质量分数分别为0.35,0.30,0.43 mg/g,即1∶2的配伍比例中儿茶素质量分数最低.结果表明当归的药味比例显著影响黄药子中主要成分的质量分数,这些质量分数变化或与当归与黄药子配伍后药效增强、毒性降低有关.

英文摘要：

This research utilizes a HPLC-UV method for determining the mass fractions of diosbulbin B and catechin in Rhizoma Dioscoreae Bulbiferae combined with different proportions of Radix Angelicae Sinensis.Linearity was obtained over the concentration range of 10～200 μg/ mL for diosbulbin B,and 200 ～ 1 200 μg/mL for catechin.The average percent recovery of diosbulbin B was 89.4％,and 92.8％ for catechin.The contents of diosbulbin B in Rhizoma Dioscoreae Bulbiferae and different combinations （1 ∶ 1,1 ∶ 2,2 ∶ 1） with Radix Angelicae Sinensis were 2.90,1.01,2.19,2.04 mg/g,respectively.The contents of catechin were 0.78,0.35,0.30,0.43 mg/g in combinations the same as above.The results suggest that different combination of Rhizoma Dioscoreae Bulbiferae and Radix Angelicae Sinensis would decide different levels of marker components in Rhizoma Dioscoreae bulbiferae,which might be relevant to the efficacy enhancement or toxicity decrease.